Document Detail


WNT/β-Catenin Signaling Pathway Stimulates the Proliferation of Cultured Adult Human Sertoli Cells via Upregulation of c-myc Expression.
MedLine Citation:
PMID:  22872488     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The role of WNT/β-catenin-signaling pathway is critical in mouse Sertoli cell maturation and tumorigenesis. This study aims to examine the effects of WNT/β-catenin signaling on the cultured adult human Sertoli cells and the underlying molecular mechanisms. Glycogen synthase kinase 3β (GSK-3β) inhibitors, SB216763 and lithium chloride (LiCl), were used to activate WNT/β-catenin-signaling pathway. 5-Bromo-2'-deoxyuridine (BrdU) incorporation assay and flow cytometry were used to analyze the proliferation and cell cycle of cultured human Sertoli cells, respectively. C-myc expression was accessed by immunofluorescence, real-time polymerase chain reaction and Western blot. The effects of c-myc on Sertoli cell proliferation were investigated by RNA interference technology and BrdU incorporation assay. The results showed activation of WNT/β-catenin signaling stimulated human Sertoli cell proliferation. Obvious increases in c-myc messenger RNA and protein expression were observed after SB216763 and LiCl treatments. Knockdown of c-myc expression attenuated the ability of WNT/β-catenin signaling to stimulate the proliferation of human Sertoli cells. WNT/β-catenin signaling enhances human Sertoli cell proliferation via upregulation of c-myc expression.
Authors:
Yi Li; Qing Gao; Gang Ying; Xiangyun Ding; Jing Hao
Related Documents :
15335218 - Fresh and cultured buccal cells as a source of mrna and protein for molecular analysis.
3169618 - Identification of transferrin receptor in cervical and endometrial tissues.
16251478 - Innate immune responses of human tracheal epithelium to pseudomonas aeruginosa flagelli...
9261468 - Trichoblastoma and basal cell carcinoma are neoplasms with follicular differentiation s...
11521698 - Migration of retinal pigment epithelial cells in vitro modulated by monocyte chemotacti...
11235908 - Atypical adenomatous hyperplasia of the lung: a probable forerunner in the development ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-7
Journal Detail:
Title:  Reproductive sciences (Thousand Oaks, Calif.)     Volume:  -     ISSN:  1933-7205     ISO Abbreviation:  Reprod Sci     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101291249     Medline TA:  Reprod Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Expression and Localization of Aquaporins 8 and 9 in Term Placenta With Oligohydramnios.
Next Document:  Expression Profile of Extracellular Matrix and Adhesion Molecules in the Development of Endometriosi...