| WNT/β-Catenin Signaling Pathway Stimulates the Proliferation of Cultured Adult Human Sertoli Cells via Upregulation of c-myc Expression. | |
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MedLine Citation:
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PMID: 22872488 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The role of WNT/β-catenin-signaling pathway is critical in mouse Sertoli cell maturation and tumorigenesis. This study aims to examine the effects of WNT/β-catenin signaling on the cultured adult human Sertoli cells and the underlying molecular mechanisms. Glycogen synthase kinase 3β (GSK-3β) inhibitors, SB216763 and lithium chloride (LiCl), were used to activate WNT/β-catenin-signaling pathway. 5-Bromo-2'-deoxyuridine (BrdU) incorporation assay and flow cytometry were used to analyze the proliferation and cell cycle of cultured human Sertoli cells, respectively. C-myc expression was accessed by immunofluorescence, real-time polymerase chain reaction and Western blot. The effects of c-myc on Sertoli cell proliferation were investigated by RNA interference technology and BrdU incorporation assay. The results showed activation of WNT/β-catenin signaling stimulated human Sertoli cell proliferation. Obvious increases in c-myc messenger RNA and protein expression were observed after SB216763 and LiCl treatments. Knockdown of c-myc expression attenuated the ability of WNT/β-catenin signaling to stimulate the proliferation of human Sertoli cells. WNT/β-catenin signaling enhances human Sertoli cell proliferation via upregulation of c-myc expression. |
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Authors:
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Yi Li; Qing Gao; Gang Ying; Xiangyun Ding; Jing Hao |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-7 |
Journal Detail:
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Title: Reproductive sciences (Thousand Oaks, Calif.) Volume: - ISSN: 1933-7205 ISO Abbreviation: Reprod Sci Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101291249 Medline TA: Reprod Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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