| Catecholamines in urine; an evaluation of alumina-trihydroxyindole methods and a description of an improved method. | |
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MedLine Citation:
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PMID: 1122637 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A critical assessment of the accuracy and practicability of five alumina-trihydroxyindole methods for the estimation of urinary catecholamines, including our proposed method, was undertaken. The recovery of noradrenaline added to urine obtained by these methods was quantitated against different types of standards, and varied from 22.9 per cent to 104.8 per cent. The major analytical problems (fluorescence suppression and loss of catecholamines during column chromatography) were evaluated and related to the recovery of the respective procedures. The improved method we developed is simple, rapid and reliable. The low fluorescence suppression and column losses of noradrenaline of this method, averaging 2.9 per cent and 5.3 per cent, respectively, resulted in a mean recovery of 92.0 per cent against an external standard. Both the chromatography step and fluorescence development are simple and short, and a batch of ten cases can be completed within 3 hours. |
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Authors:
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E S Quek; J E Buttery; G F de Witt |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 58 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1975 Jan |
Date Detail:
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Created Date: 1975-06-27 Completed Date: 1975-06-27 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 137-44 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aluminum Catecholamines / urine* Chromatography Evaluation Studies as Topic Humans Indicators and Reagents Indoles Methods Norepinephrine / urine Spectrometry, Fluorescence Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Catecholamines; 0/Indicators and Reagents; 0/Indoles; 51-41-2/Norepinephrine; 7429-90-5/Aluminum |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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