Document Detail

Catecholamine sensitivity in brown fat cells from cold-acclimated hamsters and rats.
MedLine Citation:
PMID:  6278947     Owner:  NLM     Status:  MEDLINE    
Brown fat cells, freshly isolated from cold-acclimated hamsters and rats, did not respond to norepinephrine addition with the characteristic increase in oxygen consumption (heat production) seen in cells from control animals. However, incubation of these cells for 1 h in a Krebs-Ringer bicarbonate buffer, in the presence of 10 mM pyruvate, fully restored norepinephrine responsiveness. Cells treated in this way from cold-acclimated hamsters (a hibernator) increased the rate of oxygen consumption after maximal norepinephrine stimulation as much as cells from control hamsters; also norepinephrine-stimulated fatty acid release was unaltered, indicating that brown fat cells may partly be responsible for the increase in serum fatty acid level seen during arousal from hibernation. Similarly, preincubated cells from cold-acclimated rats (a nonhibernator) increased oxygen consumption and fatty acid release as much as cells from control rats; this suggests that also in cold-acclimated rats brown fat may supply the circulation with fatty acids during cold stress. Cells from cold-acclimated animals were, however, about 10 times less sensitive to norepinephrine than cells from control animals; this desensitization may be the result of a stimulated phosphodiesterase.
J Nedergaard
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  242     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1982 Mar 
Date Detail:
Created Date:  1982-05-27     Completed Date:  1982-05-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C250-7     Citation Subset:  IM    
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MeSH Terms
Adaptation, Physiological*
Adipose Tissue, Brown / enzymology,  metabolism*
Cold Temperature*
Mesocricetus / metabolism
Norepinephrine / pharmacology*
Oxygen Consumption / drug effects
Phosphoric Diester Hydrolases / metabolism
Pyruvate Carboxylase / metabolism
Species Specificity
Reg. No./Substance:
51-41-2/Norepinephrine; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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