Document Detail


Catecholamine secretion from digitonin-treated adrenal medullary chromaffin cells.
MedLine Citation:
PMID:  6833287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The plasma membrane of cultured chromaffin cells from bovine adrenal medulla was rendered leaky by incubation in low concentrations of digitonin. Digitonin (20 microM) induced Ca2+-dependent release of 10-20% of the catecholamine in the presence of 10 microM Ca2+ without addition of secretagogue. Half-maximal catecholamine release occurred at approximately 1 microM Ca2+ x Mg2+ could not substitute for Ca2+. Cells incubated with digitonin rapidly lost their ability to exclude trypan blue. Digitonin caused release of the cytosolic markers lactic dehydrogenase and phenylethanolamine-N-methyltransferase, but, in contrast to release of catecholamine, the release of the cytosolic proteins was inhibited by Ca2+. Soluble dopamine-beta-hydroxylase, a protein marker of granule contents, was released proportionally with catecholamine in a Ca2+ dependent manner. Catecholamine release was optimal in solutions containing MgATP. Hence, digitonin-treated cells, although they lose soluble cytosolic proteins and presumably low molecular weight cytosolic constituents, maintain the Ca2+-dependent reactions of exocytosis in the presence of MgATP. Digitonin-treated chromaffin cells may be a powerful system in which to study the biochemical mechanisms underlying exocytosis.
Authors:
L A Dunn; R W Holz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  258     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-05-27     Completed Date:  1983-05-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4989-93     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adrenal Medulla / cytology*,  drug effects
Animals
Calcium / pharmacology
Carbachol / pharmacology
Cattle
Chromaffin Granules / drug effects,  metabolism*
Chromaffin System / metabolism*
Digitonin / pharmacology*
Dose-Response Relationship, Drug
Exocytosis
Norepinephrine / metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
5 RO1 AM27959/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
11024-24-1/Digitonin; 51-41-2/Norepinephrine; 51-83-2/Carbachol; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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