Document Detail

Catechol estrogen formation in the mouse uterus and its role in implantation.
MedLine Citation:
PMID:  2157614     Owner:  NLM     Status:  MEDLINE    
Estradiol-2/4-hydroxylase (E-2/4-H) activity was determined in the mouse uterus during early pregnancy as well as in ovarian steroid hormone-treated ovariectomized uterus. Under the assay conditions used, E-4-H was the predominant catechol estrogen-forming monooxygenase enzyme. The inhibition of E-4-H activity by SKF-525A, metyrapone and alpha-naphthoflavone suggested involvement of cytochrome P450-dependent monooxygenases. A haloestrogen, 2-fluoroestradiol (2-FL-E2), also inhibited this activity. During the peri-implantation period, no change in uterine E-4-H activity was noted on the morning of days 2 through 5, but the activity significantly (P less than 0.01) increased in the afternoon of day 4 of pregnancy. A single injection of estradiol-17 beta (E2, 100 ng/mouse) to ovariectomized mice significantly (P less than 0.01) elevated the level of E-4-H activity at 24 h as did injections of progesterone (P4, 2 mg/mouse) for 2 days. When 2 days of P4 (2 mg/mouse) treatment was combined with a single injection of E2 (20 ng/mouse), E-4-H activity increased 1.3-fold (P less than 0.05) by 24 h above that of P4 treatment alone. Dexamethasone (200 micrograms/mouse) and cholesterol (2 mg/mouse) treatment for 2 days had no effect on E-4-H activity. Thus, the stimulatory effect of P4 and E2 on E-4-H activity appeared to be specific. The increased activity of uterine E-4-H prior to implantation on day 4 evening and the modulation of its activity by P4 and/or E2 suggest an involvement of 4-hydroxyestradiol in embryo implantation.(ABSTRACT TRUNCATED AT 250 WORDS)
B C Paria; C Chakraborty; S K Dey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  69     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-05-21     Completed Date:  1990-05-21     Revised Date:  2014-01-08    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  25-32     Citation Subset:  IM    
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MeSH Terms
Aryl Hydrocarbon Hydroxylases*
Chromatography, High Pressure Liquid
Cytochrome P-450 CYP1A1*
Cytochrome P-450 Enzyme System / metabolism*
Dinoprostone / pharmacology
Embryo Implantation* / drug effects
Estradiol / analogs & derivatives,  pharmacology
Estrogens, Catechol / biosynthesis*
Progesterone / pharmacology
Steroid Hydroxylases / metabolism*
Uterus / metabolism*
Grant Support
Reg. No./Substance:
0/Estrogens, Catechol; 16205-32-6/2-fluoroestradiol; 4G7DS2Q64Y/Progesterone; 4TI98Z838E/Estradiol; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.-/Steroid Hydroxylases; EC Hydrocarbon Hydroxylases; EC P-450 CYP1A1; EC P-450 CYP1B1; EC 2-hydroxylase; K7Q1JQR04M/Dinoprostone

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