| Catechol concentrations in the hemolymph of the scallop, Placopecten magellanicus. | |
| | |
MedLine Citation:
|
PMID: 10753566 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Catecholamines have previously been detected in numerous tissues and are thought to control a wide variety of physiological functions in bivalve molluscs. In the present study, alumina extraction and high-performance liquid chromatography reveal the presence of significant concentrations of 3,4-dihydroxyphenylalanine (DOPA), dopamine, and 3,4-dihydroxyphenylacetic acid (DOPAC) in the hemolymph of the sea scallop, Placopecten magellanicus. The concentration of dopamine in the hemolymph averaged 223.8 ng/ml, (+/-48.4, SEM), equivalent to 10(-7) to 10(-6) M. Neither epinephrine nor norepinephrine was reliably detected in significant quantities. Previous studies have demonstrated physiological responses to dopamine with thresholds of 10(-9) to 10(-6) M, thus suggesting that this catecholamine may have an endocrine function. Furthermore, monitoring hemolymph concentrations of catechols might provide a sensitive measure of the physiological status of bivalves. For example, drugs known to affect catechol concentrations in other tissues also effect hemolymph levels. Administration of monoamine oxidase inhibitors such as pargyline, deprenyl, and clorgyline at 10(-4) M for 1 day of incubation followed by a 2-day wash resulted in decreased hemolymph concentrations of DOPAC and increased concentrations of its precursors, DOPA and dopamine. Incubation in 10(-4) M 3,5-dinitrocatechol, a catecholamine-O-methyl transferase blocker, for 1 day followed by a 2-day wash significantly increased the concentration of dopamine and DOPAC in the hemolymph. Scallops incubated in 10(-5) M alpha-methyl-p-tyrosine, a blocker of tyrosine hydroxylase, for 1 day followed by a 3-day wash in artificial seawater had significantly reduced concentrations of DOPA, dopamine, and DOPAC in the hemolymph. In addition to responding to pharmacological agents, dopamine levels also decreased significantly following thermal induction of spawning, thus suggesting that hemolymph concentrations of catechols might provide indices of reproductive activity and/or stress. |
| | |
Authors:
|
A K Pani; R P Croll |
Related Documents
:
|
1206326 - Effect of l-dopa on milk ejection and prolactin release in lactating rats. 10907726 - Chronic supranigral infusion of bdnf in normal and mptp-treated common marmosets. 9174256 - Dopamine d1 and d2 antagonists block l-dopa-induced air-stepping in decerebrate neonata... 11412836 - Bia 3-202, a novel catechol-o-methyltransferase inhibitor, enhances the availability of... 2546956 - Tumor-promoting activity and cytotoxicity of 3,4,3',4'-tetrachlorobiphenyl on n-nitroso... 3220106 - Receptor mechanisms of nicotine-induced locomotor hyperactivity in chronic nicotine-tre... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: General and comparative endocrinology Volume: 118 ISSN: 0016-6480 ISO Abbreviation: Gen. Comp. Endocrinol. Publication Date: 2000 Apr |
Date Detail:
|
Created Date: 2000-08-02 Completed Date: 2000-08-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370735 Medline TA: Gen Comp Endocrinol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 48-56 Citation Subset: IM |
Copyright Information:
|
Copyright 2000 Academic Press. |
Affiliation:
|
Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, B3H 4H7, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3,4-Dihydroxyphenylacetic Acid
/
metabolism Animals Catechol O-Methyltransferase / antagonists & inhibitors Catecholamines / metabolism* Chromatography, High Pressure Liquid Clorgyline / pharmacology Dihydroxyphenylalanine / metabolism Dopamine / metabolism Female Hemolymph / drug effects, enzymology, metabolism* Male Mollusca / metabolism* Monoamine Oxidase Inhibitors / pharmacology Pargyline / pharmacology Selegiline / pharmacology Sexual Behavior, Animal / physiology |
| Chemical | |
Reg. No./Substance:
|
0/Catecholamines; 0/Monoamine Oxidase Inhibitors; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 14611-51-9/Selegiline; 17780-72-2/Clorgyline; 555-57-7/Pargyline; 63-84-3/Dihydroxyphenylalanine; EC 2.1.1.6/Catechol O-Methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: beta-endorphin and gonadotropin-releasing hormone in the forebrain and pituitary of the female catfi...
Next Document: The distribution of lamprey GnRH-III in brains of adult sea lampreys (Petromyzon marinus).