| Catalytic peroxynitrite decomposition improves reperfusion injury after heart transplantation. | |
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MedLine Citation:
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PMID: 22401641 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Peroxynitrite, a reactive nitrogen species, has been implicated in the development of ischemia-reperfusion injury. The present study investigated the effects of the potent peroxynitrite decomposition catalyst FP15 on myocardial and endothelial function after hypothermic ischemia-reperfusion in a heterotopic rat heart transplantation model. METHODS: After a 1-hour ischemic preservation and implantation of donor hearts, reperfusion was started after application of vehicle (5% glucose solution) or FP15 (0.3 mg/kg). The assessment of left ventricular pressure-volume relations, total coronary blood flow, endothelial function, immunohistochemical markers of nitro-oxidative stress, and myocardial high-energy phosphates was performed at 1 and 24 hours of reperfusion. RESULTS: After 1 hour of reperfusion, myocardial contractility (maximal slope of systolic pressure increment at 140 μL left ventricular volume: 5435 ± 508 mm Hg/s vs 2346 ± 263 mm Hg/s), coronary blood flow (3.98 ± 0.33 mL/min/g vs 2.74 ± 0.29 mL/min/g), and endothelial function were significantly improved, nitro-oxidative stress was reduced, and myocardial high-energy phosphate content was preserved in the FP15-treated animals compared with controls. CONCLUSIONS: Pharmacologic peroxynitrite decomposition reduces reperfusion injury after heart transplantation as the result of reduction of nitro-oxidative stress and prevention of energy depletion and exerts a beneficial effect against reperfusion-induced graft cardiac and coronary endothelial dysfunction. |
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Authors:
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Gábor Szabó; Sivakkanan Loganathan; Béla Merkely; John T Groves; Matthias Karck; Csaba Szabó; Tamás Radovits |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-03-07 |
Journal Detail:
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Title: The Journal of thoracic and cardiovascular surgery Volume: 143 ISSN: 1097-685X ISO Abbreviation: J. Thorac. Cardiovasc. Surg. Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-05-18 Completed Date: 2012-07-11 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0376343 Medline TA: J Thorac Cardiovasc Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1443-9 Citation Subset: AIM; IM |
Copyright Information:
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Copyright © 2012 The American Association for Thoracic Surgery. Published by Mosby, Inc. All rights reserved. |
Affiliation:
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Department of Cardiac Surgery, University of Heidelberg, Heidelberg, Germany. gabor.szabo@urz.uni-heidelberg.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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metabolism Adenosine Monophosphate / metabolism Adenosine Triphosphate / metabolism Animals Biological Markers / metabolism Coronary Circulation / drug effects Disease Models, Animal Endothelium, Vascular / drug effects*, metabolism, physiopathology Energy Metabolism / drug effects Heart Transplantation / adverse effects* Immunohistochemistry Male Metalloporphyrins / pharmacology* Myocardial Reperfusion Injury / etiology, metabolism, physiopathology, prevention & control* Myocardium / metabolism* Oxidative Stress / drug effects Peroxynitrous Acid / metabolism* Poly Adenosine Diphosphate Ribose / metabolism Rats Rats, Inbred Lew Time Factors Tyrosine / analogs & derivatives, metabolism Vasodilation / drug effects Ventricular Function, Left / drug effects Ventricular Pressure / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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R37 GM036298/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/FeCl tetrakis-2-(triethyleneglycolmonomethylether)pyridylporphyrin; 0/Metalloporphyrins; 14691-52-2/Peroxynitrous Acid; 26656-46-2/Poly Adenosine Diphosphate Ribose; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 61-19-8/Adenosine Monophosphate |
| Comments/Corrections | |
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