Document Detail

Catalytic activity of beta-amylase from barley in different pressure/temperature domains.
MedLine Citation:
PMID:  16321045     Owner:  NLM     Status:  MEDLINE    
The depolymerization of starch by beta-amylase during exposure to hydrostatic pressure up to 700 MPa and within a temperature range from 20 to 70 degrees C has been investigated. Inactivation of the enzyme as well as alterations in conversion speed in response to combined pressure-temperature treatments were assessed by analyzing the kinetic rate constants. At 200 MPa a significant stabilization of the enzyme against heat inactivation was observed. However, high pressure also impedes the catalytic reaction and a progressive reduction of the conversion rate constants with increasing pressure was found at all temperatures investigated. For the overall reaction of maltose liberation from soluble starch in ACES buffer at pH 5.6 an optimum was identified at 106 MPa and at 63 degrees C, which is approximately 7 degrees C above the local maximum at ambient pressure (0.1 MPa). Gelatinization of nonsoluble starch granules in response to pressure-temperature (p-T) treatment has been inspected by phase-contrast microscopy and yielded circular curves of identical effect in the p-T plane.
Volker Heinz; Roman Buckow; Dietrich Knorr
Related Documents :
19420615 - Piezoresistive characteristics of mwnt nanocomposites and fabrication as a polymer pres...
16893975 - Identifying cytoplasmic input to the cell wall of growing chara corallina.
20133755 - General trend for pressurized superconducting hydrogen-dense materials.
8018705 - Red blood cell aggregability is enhanced by physiological levels of hydrostatic pressure.
3820575 - Heart rate at onset and termination of paroxysmal atrial fibrillation in apparently hea...
3592155 - Pre-oxygenation and the parturient patient.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology progress     Volume:  21     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2005 Nov-Dec
Date Detail:
Created Date:  2005-12-02     Completed Date:  2006-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1632-8     Citation Subset:  IM    
Department of Food Biotechnology and Food Process Engineering, Berlin University of Technology, Koenigin-Luise-Str. 22, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Hordeum / enzymology*
Hydrogen-Ion Concentration
Hydrostatic Pressure
Models, Biological
Starch / metabolism
beta-Amylase / antagonists & inhibitors,  metabolism*
Reg. No./Substance:
9005-25-8/Starch; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  An immobilized biotin ligase: surface display of Escherichia coli BirA on Saccharomyces cerevisiae.
Next Document:  Variables that affect xylitol production from sugarcane bagasse hydrolysate in a zeolite fluidized b...