Document Detail


Catalytic Enzyme Activity on a Biosensor Chip: Combination of Surface Plasmon Resonance and Mass Spectrometry.
MedLine Citation:
PMID:  22677626     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Surface plasmon resonance as a label-free biosensor technique has become an important tool in drug discovery campaigns in the last couple of years. For good assay performance, it is of high interest to verify the functional activity upon the immobilization of the target protein on the chip. This study illustrates the verification of the catalytic activity of the drug target protein PqsD by monitoring substrate conversion as a decrease in SPR signal and product detection by UHPLC/MS(2). This assay would be applicable to control surface activity of immobilized ligands.
Authors:
Claudia Henn; Stefan Boettcher; Anke Steinbach; Rolf W Hartmann
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-4
Journal Detail:
Title:  Analytical biochemistry     Volume:  -     ISSN:  1096-0309     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), Campus C2 3, 66123 Saarbrücken, Germany.
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