Document Detail

Catalase and estradiol inhibit mitochondrial protein S-glutathionylation.
MedLine Citation:
PMID:  22661379     Owner:  NLM     Status:  Publisher    
Regulation and downstream effects of mitochondrial protein S-glutathionylation in response to oxidative stress are poorly understood. The study aim was to determine whether anti-oxidants such as catalase and estradiol alter mitochondrial protein S-glutathionylation and in turn affect apoptosis following ultraviolet B (UV-B) light irradiation. HeLa cells were transduced with increasing amounts of adenovirus encoding catalase (Ad-Cat) and β-galactosidase (Ad-Lac Z) or pre-incubated with estradiol before induction of apoptosis by UV-B light exposure. Inhibition of mitochondrial protein S-glutathionylation was assessed using autoantibodies specific for the non-S-glutathionylated form of PDC-E2. The percentage of apoptotic cells following UV-B irradiation were not significantly different between mock cells (cells with no virus infection) and Ad-Cat and Ad-Lac Z infected cells at all viral doses (all p > 0.050). Autoantibody staining of non-S-glutathionylated PDC-E2 in apoptotic cells was three times greater in only Ad-Cat infected cells compared to only Ad-Lac Z infected cells (81.3 ± 16.7 vs 26 ± 7.2 %, respectively, p = 0.030). Similarly estradiol treatment (33 and 100 nM) also significantly increased PDC-E2 staining in apoptotic cells compared to non-treated cells (both p < 0.010). The percentage of apoptotic cells was not significantly different with any of the estradiol concentrations (all p > 0.100). The observed procaspase 12 cleavage following UV-B irradiation suggests that a mitochondrial-independent apoptotic pathway was activated. In conclusion, following an apoptotic stimulus, estradiol may inhibit mitochondrial protein S-glutathionylation without inhibiting apoptosis. This effect may play a role in ninefold greater prevalence of autoantibodies against PDC-E2 in women with primary biliary cirrhosis.
Bin Hu; Jorge Allina; Jingxiang Bai; Vivek Kesar; Joseph A Odin
Related Documents :
19774559 - Human embryonic stem cells are pre-mitotically committed to self-renewal and acquire a ...
2195549 - Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in g2.
11274069 - Regulation of a rho-associated kinase expression during the corneal epithelial cell cycle.
2298289 - The effect of quercetin on cell cycle progression and growth of human gastric cancer ce...
2328769 - A new compound which reversibly arrests t lymphocyte cell cycle near the g1/s boundary.
2002799 - Premature onset of mitosis and potentiation of chromosome damage induced by poly-d-lysi...
17936649 - Cancer: a matter of life cycle?
10023019 - The growth-inhibiting epidermal pentapeptide, pyroglu-glu-asp-ser-glyoh, inhibits growt...
1502489 - Antrectomy does not accelerate reversal of omeprazole-induced trophic effects in the ra...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-4
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  -     ISSN:  1573-4919     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Medicine, The Mount Sinai School of Medicine, Box 1133, New York, NY, 10029, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Botulinum Toxin for the Treatment of Movement Disorders.
Next Document:  Isogenic mesenchymal stem cells transplantation improves a rat model of chronic aristolochic acid ne...