Document Detail


Catalase and chondroitin sulfate derivatives against thrombotic effect induced by reactive oxygen species in a rat artery.
MedLine Citation:
PMID:  12948751     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antithrombotic activity of catalase (CAT) and chondroitin sulfate (CHS) preparations was studied in a rat model of arterial injury induced by ferrous chloride. Equal doses (according to catalytically active CAT) were used to examine the effect of native CAT, CAT-CHS covalent conjugate and mixture of native CAT and free CHS in a ratio corresponding to their contents in the conjugate. The antithrombotic activity of the derivatives was determined by the time during which arterial occlusion developed (occlusion time) and by the mass of the formed thrombus. The antithrombotic activities of the conjugate and mixture were similar and markedly higher than that of native CAT. The conjugate was more effective with respect to deceleration and prevention of arterial occlusion. Small doses of the preparations altered the structure of the formed thrombus, promoting sustained blood flow. Further investigations of the antithrombotic activity of CAT, superoxide dismutase and CHS derivatives have been outlined.
Authors:
Alexander V Maksimenko; Vladimir L Golubykh; Elena G Tischenko
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Metabolic engineering     Volume:  5     ISSN:  1096-7176     ISO Abbreviation:  Metab. Eng.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-09-01     Completed Date:  2004-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815657     Medline TA:  Metab Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  177-82     Citation Subset:  IM    
Affiliation:
Laboratory of Biochemical Engineering, Department of Biotechnology, Institute of Experimental Cardiology, Russian Cardiology Research Center, 3rd Cherepkovskaya Street 15a, 121 552 Moscow, Russia. alexmak@cardio.ru
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Flow Velocity
Carotid Artery Thrombosis / complications,  enzymology*,  pathology*,  physiopathology
Catalase / metabolism,  pharmacology*
Cerebrovascular Disorders / enzymology,  etiology,  physiopathology
Chondroitin Sulfates / metabolism,  pharmacology*
Coenzymes / metabolism,  pharmacology
Dose-Response Relationship, Drug
Drug Synergism
Male
Platelet Aggregation / drug effects
Rats
Reactive Oxygen Species / metabolism*
Chemical
Reg. No./Substance:
0/Coenzymes; 0/Reactive Oxygen Species; 9007-28-7/Chondroitin Sulfates; EC 1.11.1.6/Catalase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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