Document Detail


Catabolism of volatile sulfur compounds precursors by Brevibacterium linens and Geotrichum candidum, two microorganisms of the cheese ecosystem.
MedLine Citation:
PMID:  14580796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two Brevibacterium linens strains and the cheese-ripening yeast Geotrichum candidum were compared with regard to their ability to produce volatile sulfur compounds (VSCs) from three different precursors namely L-methionine, 4-methylthio-2-oxobutyric acid (KMBA) and 4-methylthio-2-hydroxybutyric acid (HMBA). All microorganisms were able to convert these precursors to VSCs. However, although all were able to produce VSCs from L-methionine, only G. candidum accumulated KMBA when cultivated on this amino acid, contrary to B. linens suggesting that the transamination pathway is not active in this microorganism. Conversely, a L-methionine gamma-lyase activity--which catalyses the one step L-methionine to methanethiol (MTL) degradation route--was only found in B. linens strains. Several other enzymatic activities involved in the catabolism of the precursors tested were investigated. KMBA transiently accumulated in G. candidum cultures, and was then reduced to HMBA by a KMBA dehydrogenase (KDH) activity. This activity was not detected in B. linens. Despite no HMBA dehydrogenase (HDH) was found in G. candidum, a strong HMBA oxidase (HOX) activity was measured in this microorganism. This latter activity was weakly active in B. linens. KMBA and HMBA demethiolating activities were found in all the microorganisms. Our results illustrate the metabolic diversity between cheese-ripening microorganisms of the cheese ecosystem.
Authors:
Kenza Arfi; Felix Amárita; Henry-Eric Spinnler; Pascal Bonnarme
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biotechnology     Volume:  105     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-28     Completed Date:  2004-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  245-53     Citation Subset:  IM    
Affiliation:
Department of Food and Fermentation Engineering, Institut National de la Recherche Agronomique, LGMPA, CBAI, 78850 Thiverval-Grignon, France.
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MeSH Terms
Descriptor/Qualifier:
Brevibacterium / growth & development,  metabolism*
Cheese / microbiology*
Food Microbiology
Geotrichum / growth & development,  metabolism*
Methionine / analogs & derivatives*,  metabolism
Sulfur Compounds / metabolism*
Volatilization
Chemical
Reg. No./Substance:
0/Sulfur Compounds; 583-91-5/alpha-hydroxy-gamma-methylmercaptobutyric acid; 583-92-6/2-keto-4-methylthiobutyric acid; 63-68-3/Methionine

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