| Catabolism of cysteine, cystine, cysteinesulfinate, and OTC by isolated perfused rat hindquarter. | |
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MedLine Citation:
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PMID: 8098909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The metabolism of cysteine and related compounds was investigated in the isolated perfused hindquarter of the rat. An erythrocyte-based perfusion medium was used; use of a perfluorochemical emulsion, FC-43, resulted in apparent chemical oxidation of cysteine, whereas bovine erythrocytes did not appear to contribute significantly to the metabolism of cysteine. Rat skeletal muscle perfused with L-[35S]cysteine, L-[35S]cystine, L-[35S]cysteinesulfinate, or L-2-oxo-[35S]thiazolidine-4-carboxylate (OTC) for 2 h produced [35S]sulfate and [35S]taurine. In all cases, the partitioning of cysteine or cysteinesulfinate between metabolism to taurine and sulfate was similar, suggesting that cysteine metabolism in hindquarter may occur via formation and catabolism of cysteinesulfinate by either cysteinesulfinate decarboxylase or aspartate (cysteine-sulfinate) aminotransferase. However, the activity of cysteine dioxygenase was extremely low, suggesting that the conversion of cysteine to cysteinesulfinate may have been non-enzymatic. |
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Authors:
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J L Ensunsa; L L Hirschberger; M H Stipanuk |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 264 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1993 May |
Date Detail:
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Created Date: 1993-06-22 Completed Date: 1993-06-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E782-9 Citation Subset: IM |
Affiliation:
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Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenine Nucleotides
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metabolism Animals Cysteine / analogs & derivatives*, metabolism* Cystine / metabolism* Hindlimb Lactates / metabolism Male Muscles / metabolism* Neurotransmitter Agents / metabolism Perfusion Phosphocreatine / metabolism Pyrrolidonecarboxylic Acid Pyruvates / metabolism Radioisotope Dilution Technique Rats Rats, Sprague-Dawley Sulfates / metabolism Sulfur Radioisotopes Taurine / metabolism Thiazoles / metabolism* Thiazolidines |
| Chemical | |
Reg. No./Substance:
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0/Adenine Nucleotides; 0/Lactates; 0/Neurotransmitter Agents; 0/Pyruvates; 0/Sulfates; 0/Sulfur Radioisotopes; 0/Thiazoles; 0/Thiazolidines; 107-35-7/Taurine; 19750-45-9/2-oxothiazolidine-4-carboxylic acid; 2381-08-0/cysteine sulfinic acid; 52-90-4/Cysteine; 56-89-3/Cystine; 67-07-2/Phosphocreatine; 98-79-3/Pyrrolidonecarboxylic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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