| Catabolism of arginine and ornithine in the perfused rat liver: effect of dietary protein and of glucagon. | |
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MedLine Citation:
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PMID: 10710507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rates of oxidation of arginine and ornithine that occurred through a reaction pathway involving the enzyme ornithine aminotransferase (EC 2.6.1.13) were determined using (14)C-labeled amino acids in the isolated nonrecirculating perfused rat liver. At physiological concentrations of these amino acids, their catabolism is subject to chronic regulation by the level of protein consumed in the diet. (14)CO(2) production from [U-(14)C]ornithine (0.1 mM) and from [U-(14)C]arginine (0.2 mM) was increased about fourfold in livers from rats fed 60% casein diets for 3-4 days. The catabolism of arginine in the perfused rat liver, but not that of ornithine, is subject to acute regulation by glucagon (10(-7) M), which stimulated arginine catabolism by approximately 40%. Dibutyryl cAMP (0.1 mM) activated arginine catabolism to a similar extent. In retrograde perfusions, glucagon caused a twofold increase in the rate of arginine catabolism, suggesting an effect of glucagon on arginase in the perivenous cells. |
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Authors:
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D O'Sullivan; J T Brosnan; M E Brosnan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 278 ISSN: 0193-1849 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2000 Mar |
Date Detail:
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Created Date: 2000-05-24 Completed Date: 2000-05-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E516-21 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Animals Arginine / metabolism* Bucladesine / pharmacology Caseins / pharmacology Dietary Proteins / pharmacology Dose-Response Relationship, Drug Glucagon / pharmacology Liver / drug effects, metabolism* Male Ornithine / metabolism* Ornithine-Oxo-Acid Transaminase / metabolism Perfusion Rats Rats, Sprague-Dawley Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Caseins; 0/Dietary Proteins; 362-74-3/Bucladesine; 7006-33-9/Ornithine; 74-79-3/Arginine; 9007-92-5/Glucagon; EC 2.6.1.13/Ornithine-Oxo-Acid Transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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