| Catabolism of the apoprotein of low density lipoproteins by the isolated perfused rat liver. | |
| | |
MedLine Citation:
|
PMID: 209115 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Isolated rat livers were perfused for 4 hours in a recirculating system containing washed rat erythrocytes. Biologically screened, radioiodinated low density lipoproteins (1.030 < d < 1.055 g/ml) were added to the perfusate with different amounts of whole serum to supply unlabeled rat low density lipoproteins. Apolipoprotein B contained 90% of the bound (131)I, other apolipoproteins contained 4%, and lipids contained the remainder. The fraction of apolipoprotein mass degraded during the perfusion was quantified by the linear increment of non-protein-bound radioiodine in the perfusate, corrected for the increment observed during recirculation of the perfusate in the absence of a liver. The fractional catabolic rate ranged from 0.3 to 1.7%/hr in seven experiments and was inversely related to the size of perfusate pool of low density apolipoprotein. The catabolic rate of low density apolipoprotein (fractional catabolic rate x pool size) in four livers, in which the concentration of rat low density lipoproteins was 50-100% of that present in intact rats, was 5.3 +/- 2.7 micro g hr(-1) (mean +/- SD). Similar results were obtained with human low density lipoproteins. These rates were compared with catabolic rates for the apoprotein of rat low density lipoproteins in intact animals. Fractional catabolic rate in vivo, obtained by multi-compartmental analysis of the disappearance curve of (131)I-labeled low density apolipoprotein from blood plasma, was 15.2 +/- 3.1% hr(-1) (mean +/- SD). Total catabolic rate in vivo (fractional catabolic rate x intravascular pool of low density apolipoprotein) was 76 +/- 14 micro g hr(-1) (mean +/- SD). The results suggest that only a small fraction of low density apolipoprotein mass in rats is degraded by the liver. |
| | |
Authors:
|
G Sigurdsson; S P Noel; R J Havel |
Related Documents
:
|
2630315 - Effect of glucagon on glucose output from bivascularly perfused rat liver. 3745435 - Inhibition of lactate removal by ketone bodies in rat liver. evidence for a quantitativ... 7177775 - Dysfunction of the adrenergic system in phosphate depleted rats. 6768355 - Degradation of [3h]chondroitin 4-sulphate and re-utilization of the [3h]hexosamine comp... 7908295 - Autocrine regulation of rat chondrocyte proliferation by natriuretic peptide c and its ... 8084455 - Evaluation of an experimental rat model for peritoneal dialysis: fluid and solute trans... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Journal of lipid research Volume: 19 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1978 Jul |
Date Detail:
|
Created Date: 1978-09-30 Completed Date: 1978-09-30 Revised Date: 2007-03-23 |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 628-34 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apolipoproteins / blood, metabolism* Iodine Radioisotopes Isotope Labeling Lipoproteins, LDL / blood, metabolism* Liver / metabolism* Male Perfusion Rats |
| Chemical | |
Reg. No./Substance:
|
0/Apolipoproteins; 0/Iodine Radioisotopes; 0/Lipoproteins, LDL |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Relationship between the tissue level of cyclic AMP and the fat cell size of human adipose tissue.
Next Document: Uptake and degradation of high density lipoprotein: comparison of fibroblasts from normal subjects a...