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Catabolism of branched-chain amino acids in heart failure: insights from genetic models.
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MedLine Citation:
PMID:  21210099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genetic defects in amino acid metabolism are major causes of newborn diseases that often lead to abnormal development and function of the central nervous system. Their direct impact on cardiac development and function has rarely been investigated. Recently, the authors have established that a mitochondrial targeted 2C-type ser/thr protein phosphatase, PP2Cm, is the endogenous phosphatase of the branched-chain alpha keto acid-dehydrogenase complex (BCKD) and functions as a key regulator in branched-chain amino acid catabolism and homeostasis. Genetic inactivation of PP2Cm in mice leads to significant elevation in plasma concentrations of branched-chain amino acids and branched-chain keto acids at levels similar to those associated with intermediate mild forms of maple syrup urine disease. In addition to neuronal tissues, PP2Cm is highly expressed in cardiac muscle, and its expression is diminished in a heart under pathologic stresses. Whereas phenotypic features of heart failure are seen in PP2Cm-deficient zebra fish embryos, cardiac function in PP2Cm-null mice is compromised at a young age and deteriorates faster by mechanical overload. These observations suggest that the catabolism of branched-chain amino acids also has physiologic significance in maintaining normal cardiac function. Defects in PP2Cm-mediated catabolism of branched-chain amino acids can be a potential novel mechanism not only for maple syrup urine disease but also for congenital heart diseases and heart failure.
Authors:
Haipeng Sun; Gang Lu; Shuxun Ren; Jaunian Chen; Yibin Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-01-07
Journal Detail:
Title:  Pediatric cardiology     Volume:  32     ISSN:  1432-1971     ISO Abbreviation:  Pediatr Cardiol     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-09     Completed Date:  2011-08-18     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  8003849     Medline TA:  Pediatr Cardiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-10     Citation Subset:  IM    
Affiliation:
Division of Molecular Medicine, Department of Anesthesiology, Molecular Biology Institute, Cardiovascular Research Laboratories, David Geffen School of Medicine, CSH, Room BH 569, 650 Charles E. Young Drive, Los Angeles, CA 90095, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids, Branched-Chain / genetics,  metabolism*
Animals
Heart Defects, Congenital / metabolism*
Heart Failure / genetics,  metabolism*
Humans
Mice
Models, Genetic
Protein Phosphatase 2
Grant Support
ID/Acronym/Agency:
HL080111/HL/NHLBI NIH HHS; HL088640/HL/NHLBI NIH HHS; HL098954/HL/NHLBI NIH HHS; HL103205/HL/NHLBI NIH HHS; HL70079/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids, Branched-Chain; EC 3.1.3.16/Protein Phosphatase 2
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Pediatr Cardiol
ISSN: 0172-0643
ISSN: 1432-1971
Publisher: Springer-Verlag, New York
Article Information
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© The Author(s) 2010
Received Day: 29 Month: 11 Year: 2010
Accepted Day: 3 Month: 12 Year: 2010
Electronic publication date: Day: 7 Month: 1 Year: 2011
pmc-release publication date: Day: 7 Month: 1 Year: 2011
Print publication date: Month: 3 Year: 2011
Volume: 32 Issue: 3
First Page: 305 Last Page: 310
ID: 3051105
PubMed Id: 21210099
Publisher Id: 9856
DOI: 10.1007/s00246-010-9856-9

Catabolism of Branched-Chain Amino Acids in Heart Failure: Insights from Genetic Models
Haipeng SunAff1
Gang LuAff1
Shuxun RenAff1
Jaunian ChenAff1
Yibin WangAff1 Address: yibinwang@mednet.ucla.edu
Division of Molecular Medicine, Departments of Anesthesiology, Medicine and Physiology, Molecular Biology Institute, Cardiovascular Research Laboratories, David Geffen School of Medicine, CSH, Room BH 569, 650 Charles E. Young Drive, Los Angeles, CA 90095 USA

Amino Acid Metabolism and Genetic Disorders

Amino acids are key nutrient molecules essential for cell growth, survival, and normal function. In addition to providing building blocks for protein synthesis, many amino acids are an essential ingredient for biosynthesis of other molecules as the sources of nitrogen and carbon [8]. Some amino acids, including branched-chain amino acids, also have been shown to possess a potent signaling function to regulate global growth and metabolism [2, 13, 14, 17, 18, 22, 23]. Therefore, the impact of amino acid metabolism on embryonic development and human congenital diseases has long been recognized.

Maple syrup urine disease, one of the most common genetic disorders caused by defects in amino acid metabolism, is a target of mandatory newborn screening in most of the United States and throughout the world [5, 10, 20]. The clinical manifestations of classical maple syrup urine disease are mostly neuropathologic, including seizure and mental retardation [7]. However, recent studies from animal models of maple syrup urine disease raise concerns about the potential adverse impact of branched-chain amino acid metabolic defects on cardiac development and function. These concerns are the focus of the discussion in this review.


Catabolism of Branched-Chain Amino Acids

Branched-chain amino acids (BCAAs) including leucine, isoleucine, and valine are essential amino acids that must be acquired from external food. In addition to their abundant presence in protein peptides as key hydrophobic building blocks, BCAAs also serve as significant sources for biosynthesis of sterol, keto bodies, and glucose [3]. Among the BCAAs, particularly leucine has potent signaling activity to promote protein synthesis, cellular metabolism, and cell growth in a mammalian target of rapamycin (mTOR)-dependent manner [4, 15, 19].

Although BCAAs are necessary for normal growth and function at cellular and organism levels, an excess amount of free BCAAs also can be pathologic. A high plasma level of BCAAs is the benchmark and cause of maple syrup urine disease, a potentially life-threatening disorder affecting 1 of 180,000 newborn babies on the average in the general population, with a much higher prevalence among certain Amish, Mennonite, and Jewish communities [5, 20].

Because BCAAs are essential amino acids with no biosynthesis pathways in animal cells, their homeostasis is determined largely by catabolic activities in a number of organs, particularly the liver [9]. The first step in the catabolism of BCAAs is carried out in brain, muscle, and many nonhepatic tissues by the branched-chain aminotransferase (BCAT) to convert BCAAs into branched-chain alpha keto acids (BCKAs) [9]. The BCKAs are decarboxylated by the branched-chain alpha keto acid dehydrogenase (BCKD) complex in the liver as well as other tissues and eventually degraded into acetyl-coenzyme A (CoA) or succinyl-CoA to fuel the TCA cycle. As BCKD mediates this rate-limiting step in BCAA catabolism, its activity dictates the steady-state levels of BCAA and BCKA. It thus is a target of multiple regulatory mechanisms, including cyclic adenosine monophosphate (cAMP)-mediated induction of RNA transcription and phosphorylation-mediated inhibition/activation of its enzymatic activity.

The BCKD complex is genetically linked with the pyruvate dehydrogenase complex (PDH) because they share extensive homology in their subunit composition and regulation. Like PDH, BCKD holoenzyme contains 24 copies of catalytic subunits E2/E3 and an equal number of regulatory subunits E1α and E1β. At low BCAA levels, E1α is hyperphosphorylated by BCKD kinase, leading to lower BCKD activity and reduced loss of BCAA. At a high BCAA level, E1α is dephosphorylated by BCKD phosphatase, leading to induced BCKD activity and the removal of excess BCAA. Therefore, BCKD phosphorylation/dephosphorylation is critical to BCAA homeostasis [9].


Molecular Identification of BCKD Phosphatase

Based on the importance of BCKD phosphorylation in its regulation, BCKD phosphatase has been well established as a key regulator in BCAA catabolism. However, its molecular identity has remained elusive for decades. Recently, through genome scanning, we discovered a mitochondrial targeted 2C-type ser/thr protein phosphatase that we named PP2C in mitochondria (PP2Cm) [11].

Through extensive proteomic and biochemical analysis, we established that PP2Cm is the missing BCKD phosphatase responsible for BCAA-induced dephosphorylation and activation of BCKD activity [12]. This conclusion is based on the following lines of evidence: (1) BCKD subunits, including E2 and E1α, are specifically associated with PP2Cm in vitro and in vivo; (2) PP2Cm expression effectively dephosphorylates E1α Ser-293 phosphorylation, a key residue in BCKD enzymatic regulation; (3) PP2Cm-deficient cells have elevated basal E1α phosphorylation, which remains high at BCKA treatment; (4) PP2Cm-deficient mice have a significantly higher plasma level of BCAA and BCKA with impaired BCAA clearance after a high dose of BCAA ingestion; and (5) PP2Cm-deficient newborn mice have significantly higher mortality under a high-protein diet challenge.

All these data establish that PP2Cm is the endogenous BCKD phosphatase with an essential function in BCAA catabolism. The analysis with PP2Cm KO mice indicates that a defect in PP2Cm is a potential novel mechanism for the intermediate/inducible forms of maple syrup urine disease.


PP2Cm in Cardiac Regulation

The discovery of PP2Cm as the BCKD phosphatase and a key regulator in BCAA metabolism as well as the establishment of PP2Cm-deficient zebra fish and mouse models offers an opportunity to investigate the impact of BCAA regulation in intact animals. In both zebra fish embryos and adult mice, PP2Cm is highly expressed in both cardiac muscle and the central nervous system (Figs. 1, 2). Its expression in the heart is dynamically regulated by stress, as measured by both mRNA and protein levels, with significantly reduced levels in hypertrophic and failing hearts [11].

It is not clear whether loss of PP2Cm is a contributing factor to cardiac pathology or simply a phenomenon associated with the diseased hearts. To investigate this question, we analyzed the cardiac performance in PP2Cm-deficient zebra fish and mice. Using morpholingoes specifically targeted to zPP2Cm translation initiation codon (ATG-MO), we demonstrated that. PP2Cm-deficient fish embryos displayed a dose-dependent loss of cardiac contractility and that most of them did not survive beyond the early embryonic stage [11]. Accelerated heart failure developed in PP2Cm-deficient mice after mechanical overload induced by transaortic constriction (Fig. 3).

These evidences suggest that loss of PP2Cm is not a mere consequence but rather a significant contributor to the pathogenesis of heart failure. These studies for the first time implicated BCAA catabolism also as an important aspect of cardiac pathophysiology and showed that defects in BCAA homeostasis can have a significant adverse impact on cardiac function and disease progression.

The underlying mechanisms for the clinical manifestations of maple syrup urine disease still are not well established, and the adverse impact on glutamine transport and the induction of reactive-oxygen species (ROS) have been implicated [1, 6, 24]. In our own studies with cardiac tissue or cultured cardiomyocytes, we also explored a number of these possible mechanisms.

  1. We examined the cytotoxic effect of the accumulated BCAA/BCKA on cadiomyocyte survival, demonstrating that PP2Cm-deficient mice have a significantly elevated ROS level and are more susceptible to calcium-induced permeability transition pore opening in mitochondria [11]. Whether and how this effect is caused by accumulated BCAA or BCKA remains to be investigated further. However, a clear implication of the observed cytotoxicity is more cell death. Indeed, we observed that PP2Cm deficiency can lead to more apoptotic cell death in both developing embryos and cultured myocytes.
  2. Metabolic effects of accumulated BCAA/BCKA can be pathologic in the heart. It is known that BCAA/BCKA can inhibit pyruvate and fatty acid transport and utilization [16, 19]. Because cardiac tissue has a constant high demand for pyruvate and fatty acid as its main fuel source, chronically elevated BCAA/BCKA can potentially block normal bioenergenic homeostasis in the heart, leading to contractile dysfunction and accelerated heart failure under increased mechanical load or aging. Although we observed significant changes in a number of metabolic genes in PP2Cm-deficient hearts (Lu et al., unpublished results), a direct analysis of cardiac metabolic status and energy consumption is needed to support this hypothesis.
  3. Our findings showed that PP2Cm mediated direct modification of mitochondrial function in cellular bioenergenics and survival. Although BCKD is the only substrate identified for PP2Cm to date, it is possible that other uncharacterized substrates in mitochondria contribute to PP2Cm-mediated signaling. This is supported by our observation that the isolated PP2Cm-deficient mitochondria have abnormal sensitivity to calcium-induced permeability transition pore opening even in the absence of BCAA or BCKA treatment [11]. The molecular basis of this observation remains unclear, but it may imply that branched-amino acid metabolism is functionally coupled with mitochondrial inner membrane permeability, which has a key role in mitochondrial calcium, respiration coupling, ROS, and cellular viability.


Perspectives

Branched-chain amino acids are important nutrient molecules with a potent signaling effect. Free BCAAs and their catabolic intermediates, BCKAs, are tightly maintained in animals by a highly regulated catabolic pathway. Defects in catabolism of BCAAs cause maple syrup urine disease, one of the most common metabolic disorders in the human population.

Although most clinical features of maple syrup urine disease are neurologic, our recent findings from cellular studies and animal models suggest that missing a key regulator in the catabolism of BCAAs also can cause a significant impairment in cardiac function (Fig. 4). The implication of this observation remains to be further established. However, a recent study based on metabolic profiling of peripheral blood has demonstrated a link between abnormal metabolism of BCAAs and coronary diseases [21]. As the complete molecular components of BCAA catabolic pathways are being discovered, better diagnosis will be available to identify patients with nonclassic, intermediate, or inducible maple syrup urine disease based on sequencing evidence. The insights learned from our study argue strongly for a better understanding of the role that the catabolism of BCAAs plays in the heart and its potential impact on cardiac pathology in addition to its damage to the central nervous system.


This work was supported in part by National Institutes of Health grants HL70079, HL103205, HL098954, HL080111, and HL088640. Yibin Wang is an established investigator of the American Heart Association. Haipeng Sun is a recipient of a postdoctoral fellowship and Gang Lu a recipient of predoctoral fellowship awards from the Great Western Affiliate of American Heart Association.

Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.


References
1.. Amaral AU,Leipnitz G,Fernandes CG,Seminotti B,Schuck PF,Wajner M. Alpha-ketoisocaproic acid and leucine provoke mitochondrial bioenergetic dysfunction in rat brainBrain ResYear: 20101324758410.1016/j.brainres.2010.02.01820153737
2.. Avruch J,Long X,Ortiz-Vega S,Rapley J,Papageorgiou A,Dai N. Amino acid regulation of TOR complex 1Am J Physiol Endocrinol MetabYear: 2009296E592E60210.1152/ajpendo.90645.200818765678
3.. Baquet A,Lavoinne A,Hue L. Comparison of the effects of various amino acids on glycogen synthesis, lipogenesis, and ketogenesis in isolated rat hepatocytesBiochem JYear: 1991273Pt 157621989589
4.. Chotechuang N,Azzout-Marniche D,Bos C,Chaumontet C,Gausseres N,Steiler T,Gaudichon C,Tome D. mTOR, AMPK, and GCN2 coordinate the adaptation of hepatic energy metabolic pathways in response to protein intake in the ratAm J Physiol Endocrinol MetabYear: 2009297E1313E132310.1152/ajpendo.91000.200819738034
5.. Chuang DT. Maple syrup urine disease: it has come a long wayJ PediatrYear: 1998132S17S2310.1016/S0022-3476(98)70523-29546032
6.. Contrusciere V,Paradisi S,Matteucci A,Malchiodi-Albedi F. Branched-chain amino acids induce neurotoxicity in rat cortical culturesNeurotox ResYear: 20101739239810.1007/s12640-009-9115-019763733
7.. Fernstrom JD. Branched-chain amino acids and brain functionJ NutrYear: 20051351539S1546S15930466
8.. Harper AE,Miller RH,Block KP. Branched-chain amino acid metabolismAnnu Rev NutrYear: 1984440945410.1146/annurev.nu.04.070184.0022056380539
9.. Harris RA,Joshi M,Jeoung NH. Mechanisms responsible for regulation of branched-chain amino acid catabolismBiochem Biophys Res CommunYear: 200431339139610.1016/j.bbrc.2003.11.00714684174
10.. Kaye CI,Accurso F,Franchi S,Lane PA,Hope N,Sonya P,GB S,Michele AL. Newborn screening fact sheetsPediatricsYear: 2006118e934e96310.1542/peds.2006-178316950973
11.. Lu G,Ren S,Korge P,Choi J,Dong Y,Weiss J,Koehler C,Chen JN,Wang Y. A novel mitochondrial matrix serine/threonine protein phosphatase regulates the mitochondria permeability transition pore and is essential for cellular survival and developmentGenes DevYear: 20072178479610.1101/gad.149910717374715
12.. Lu G,Sun H,She P,Youn JY,Warburton S,Ping P,Vondriska TM,Cai H,Lynch CJ,Wang Y. Protein phosphatase 2Cm is a critical regulator of branched-chain amino acid catabolism in mice and cultured cellsJ Clin InvestYear: 20091191678168710.1172/JCI3815119411760
13.. Marc Rhoads J,Wu G. Glutamine, arginine, and leucine signaling in the intestineAmino AcidsYear: 20093711112210.1007/s00726-008-0225-419130170
14.. Meijer AJ. Amino acid regulation of autophagosome formationMethods Mol BiolYear: 20084458910910.1007/978-1-59745-157-4_518425444
15.. Newgard CB,An J,Bain JR,Muehlbauer MJ,Stevens RD,Lien LF,Haqq AM,Shah SH,Arlotto M,Slentz CA,Rochon J,Gallup D,Ilkayeva O,Wenner BR,Yancy WS Jr,Eisenson H,Musante G,Surwit RS,Millington DS,Butler MD,Svetkey LP. A branched-chain amino acid-related metabolic signature that differentiates obese and lean humans and contributes to insulin resistanceCell MetabYear: 2009931132610.1016/j.cmet.2009.02.00219356713
16.. Nishimura J,Masaki T,Arakawa M,Seike M,Yoshimatsu H. Isoleucine prevents the accumulation of tissue triglycerides and upregulates the expression of PPARalpha and uncoupling protein in diet-induced obese miceJ NutrYear: 201014049650010.3945/jn.109.10897720089773
17.. Potier M,Darcel N,Tome D. Protein, amino acids, and the control of food intakeCurr Opin Clin Nutr Metab CareYear: 200912545810.1097/MCO.0b013e32831b9e0119057188
18.. Rennie MJ,Bohe J,Smith K,Wackerhage H,Greenhaff P. Branched-chain amino acids as fuels and anabolic signals in human muscleJ NutrYear: 2006136264S268S16365095
19.. Saha AK,Xu XJ,Lawson E,Deoliveira R,Brandon AE,Kraegen EW,Ruderman NB. Downregulation of AMPK accompanies leucine- and glucose-induced increases in protein synthesis and insulin resistance in rat skeletal muscleDiabetesYear: 201059102426243410.2337/db09-187020682696
20.. Schadewaldt P,Wendel U. Metabolism of branched-chain amino acids in maple syrup urine diseaseEur J PediatrYear: 1997156Suppl 1S62S6610.1007/PL000142749266218
21.. Shah SH,Bain JR,Muehlbauer MJ,Stevens RD,Crosslin DR,Haynes C,Dungan J,Newby LK,Hauser ER,Ginsburg GS,Newgard CB,Kraus WE. Association of a peripheral blood metabolic profile with coronary artery disease and risk of subsequent cardiovascular eventsCirc Cardiovasc GenetYear: 2010320721410.1161/CIRCGENETICS.109.85281420173117
22.. Stipanuk MH. Leucine and protein synthesis: mTOR and beyondNutr RevYear: 20076512212910.1111/j.1753-4887.2007.tb00289.x17425063
23.. Vary TC,Lynch CJ. Nutrient-signaling components controlling protein synthesis in striated muscleJ NutrYear: 20071371835184317634251
24.. Yudkoff M,Daikhin Y,Nissim I,Horyn O,Luhovyy B,Lazarow A. Brain amino acid requirements and toxicity: the example of leucineJ NutrYear: 20051351531S1538S15930465

Figures

[Figure ID: Fig1]
Fig. 1 

Expression pattern of PP2C in mitochondria (PP2Cm) of developing zebra fish embryos. a–fIn situ hybridization signal of fPP2Cm mRNA in developing zebra fish embryos at a specific stage as indicated (hpf: hour postfertilization). g Transgene construct of fPP2Cm promoter driving GFP expression. h GFP signal observed under ultraviolet illumination in transgenic zebrafish at 72 hpf stage under different magnifications. Arrows indicate heart tube



[Figure ID: Fig2]
Fig. 2 

Loss of fPP2Cm expression leads to heart failure in zebra fish embryo. a Video frames of cmlc-GFP transgenic fish embryo recorded under ultraviolet illumination after treatment with different concentrations of PP2Cm morpholigo. b M-mode image of zebra fish hearts from line-scanning analysis as shown in A as well as heart rate (HR) and percentile fractional shortening (FS%) measurements. Adapted from Lu et al. [11] with permission



[Figure ID: Fig3]
Fig. 3 

Expression and function of PP2Cm in the heart. a Expression of PP2Cm in the heart illustrated by positive Lac-Z staining in a PP2Cm+/lacZ heart. b Representative echocardiogram of a wild type and a PP2Cm KO heart before and after 8 weeks of pressure overload induced by TA



[Figure ID: Fig4]
Fig. 4 

Potential role played by catabolism of branched-chain amino acids (BCAA)/branched-chain alpha keto acids (BCKA) in heart failure. Schematic illustration showing the potential negative impact of an elevated BCAA/BCKA level on cardiac function via its regulation of mitochondrial electrophone transfer chain (ETC), reactive oxygen species (ROS), and mitochondrial permeability transition pore (mPTP)



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Keywords: Keywords Amino acid metabolism, Branched-chain amino acid catabolism, Branched-chain alpha keto acid-dehydrogenase complex, PP2Cm.

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