| Caspase-resistant vimentin suppresses apoptosis after photodynamic treatment with a silicon phthalocyanine in Jurkat cells. | |
| | |
MedLine Citation:
|
PMID: 11368515 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Oxidative stress, such as photodynamic therapy, is an apoptosis inducer. Apoptosis, as well as photosensitization, have been associated with disruption of the cytoskeletal network. The purpose of the present study was to assess the role of vimentin, a major cytoskeletal protein, in apoptosis after photodynamic treatment (PDT) with the silicon phthalocyanine Pc 4 in human Jurkat T cells. Here we show for the first time that photosensitization with Pc 4 initiates vimentin cleavage and that this event precedes poly(ADP-ribose) polymerase (PARP) degradation. Similar findings were obtained in the presence of C2-ceramide, an inducer of oxidative stress and apoptosis. In the presence of benzyloxycarbonyl-Val-Ala-Asp(O-methyl)-fluoromethylketone, a pan-caspase inhibitor, Pc 4-PDT-induced vimentin and PARP cleavage were abolished. In Jurkat cells transfected with a caspase-resistant vimentin apoptosis was partly suppressed and delayed post-Pc 4-PDT. We suggest that the full-length vimentin confers resistance to nuclear apoptosis after PDT with Pc 4. |
| | |
Authors:
|
I Belichenko; N Morishima; D Separovic |
Related Documents
:
|
11330665 - Involvement of caspase-3 in apoptosis induced by viscum album var. coloratum agglutinin... 19356725 - Downregulation of plk-1 expression in kaempferol-induced apoptosis of mcf-7 cells. 9050895 - Inhibition of interleukin 1beta converting enzyme family proteases reduces ischemic and... 10200555 - Emerging roles of caspase-3 in apoptosis. 22052905 - Autophagy induced by deficiency of sphingosine-1-phosphate phosphohydrolase 1 is switch... 17950395 - Exposure to inorganic mercury in vivo attenuates extrinsic apoptotic signaling in staph... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Archives of biochemistry and biophysics Volume: 390 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2001 Jun |
Date Detail:
|
Created Date: 2001-05-22 Completed Date: 2001-06-28 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
|
Languages: eng Pagination: 57-63 Citation Subset: IM |
Copyright Information:
|
Copyright 2001 Academic Press. |
Affiliation:
|
Department of Radiation Oncology, Case Western Reserve University School of Medicine, Cleveland, Ohio, 44106-4942, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Caspases / pharmacology Drug Resistance Humans Indoles / pharmacology* Jurkat Cells Organosilicon Compounds / pharmacology* Oxidative Stress Photochemotherapy Photosensitizing Agents / pharmacology* Poly(ADP-ribose) Polymerases / metabolism Silanes* Sphingosine / analogs & derivatives, pharmacology Transfection Vimentin / genetics, metabolism, pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
|
R29 CA77475/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Indoles; 0/N-acetylsphingosine; 0/Organosilicon Compounds; 0/Photosensitizing Agents; 0/Silanes; 0/Vimentin; 123-78-4/Sphingosine; 135719-28-7/silicon phthalocyanine; 92396-88-8/bis(tri-n-hexylsiloxy)(2,3-naphthalocyaninato)silicon; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Type I collagen stabilization of matrix metalloproteinase-2.
Next Document: Short heterodimer partner (SHP) orphan nuclear receptor inhibits the transcriptional activity of ary...