| Caspase-independent apoptosis, in human MCF-7c3 breast cancer cells, following photodynamic therapy, with a novel water-soluble phthalocyanine. | |
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MedLine Citation:
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PMID: 20382257 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new water-soluble phthalocyanine derivative, 2,3,9,10,16,17,23,24-octakis(3-aminopropyloxy) phthalocyaninato zinc II (PoII) was studied as a photosensitizer for photodynamic therapy (PDT) in MCF-7c3 cells. We report here that PoII and red light induces apoptosis. However, the precise mechanism appears to differ from that induced by PDT with other known phthalocyanines. The present study provides evidence that in the case of PoII, caspases do not participate in the apoptotic response. PoII-PDT-treated cells exhibited chromatin condensation and phosphatidylserine (PS) externalization. In the absence of light activation, PoII had no detectable cytotoxic effect. An early event upon PoII-PDT was photodamage to lysosomes, suggesting that they are the primary sites of action. Moreover, the treatment induces Bid activation, mitochondrial swelling and translocation of apoptosis-inducing factor (AIF) to the nucleus. An atypical proteolysis of poly(ADP-ribose) polymerase (PARP) indicative of calpain-like activation was observed. These data support the notion that an alternative mechanism of caspase-independent apoptosis was found in PoII-photosensitized cells. |
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Authors:
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Natalia B Rumie Vittar; Josefina Awruch; Kashif Azizuddin; Viviana Rivarola |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-09 |
Journal Detail:
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Title: The international journal of biochemistry & cell biology Volume: 42 ISSN: 1878-5875 ISO Abbreviation: Int. J. Biochem. Cell Biol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-02 Completed Date: 2010-09-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508482 Medline TA: Int J Biochem Cell Biol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1123-31 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Departamento Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Ruta 8 Km 601, Río Cuarto, Argentina. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Apoptosis Inducing Factor / metabolism BH3 Interacting Domain Death Agonist Protein / metabolism Breast Neoplasms / drug therapy*, enzymology, pathology* Calpain / metabolism Caspases / metabolism Cell Line, Tumor Chromatin / metabolism DNA Fragmentation / drug effects DNA, Neoplasm / metabolism Enzyme Activation / drug effects Female G1 Phase / drug effects Humans Indoles / pharmacology*, therapeutic use* Lysosomes / drug effects, metabolism Mitochondrial Swelling / drug effects Organometallic Compounds / pharmacology*, therapeutic use* Phosphatidylserines / metabolism Photochemotherapy* Photosensitizing Agents / pharmacology, therapeutic use Protein Transport / drug effects Solubility / drug effects Water / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Inducing Factor; 0/BH3 Interacting Domain Death Agonist Protein; 0/Chromatin; 0/DNA, Neoplasm; 0/Indoles; 0/Organometallic Compounds; 0/Phosphatidylserines; 0/Photosensitizing Agents; 0/ZnPc(COOH)16; 7732-18-5/Water; EC 3.4.22.-/Calpain; EC 3.4.22.-/Caspases |
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