Document Detail

Caspase-dependent inhibition of store-operated Ca(2+) entry into apoptosis-committed Jurkat cells.
MedLine Citation:
PMID:  20643097     Owner:  NLM     Status:  MEDLINE    
Activation of T-cells triggers store-operated Ca(2+) entry, which begins a signaling cascade leading to induction of appropriate gene expression and eventually lymphocyte proliferation and differentiation. The simultaneous enhancement of Fas ligand gene expression in activated cells allows the immune response to be limited by committing the activated cells to apoptosis. In apoptotic cells the store-operated calcium entry is significantly inhibited. It has been documented that moderate activation of Fas receptor may cause reversible inhibition of store-operated channels by ceramide released from hydrolyzed sphingomyelin. Here we show that activation of Fas receptor in T-cells results in caspase-dependent decrease of cellular STIM1 and Orai1 protein content. This effect may be responsible for the substantial inhibition of Ca(2+) entry into Jurkat cells undergoing apoptosis. In turn, this inhibition might prevent overloading of cells with calcium and protect them against necrosis.
Marta Onopiuk; Katarzyna Wierzbicka; Wojciech Brutkowski; Joanna Szczepanowska; Krzysztof Zabłocki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-17
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  399     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-09-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  198-202     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Department of Biochemistry, The Nencki Institute of Experimental Biology, Warsaw, Poland.
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MeSH Terms
Antibodies / immunology
Antigens, CD95 / agonists,  metabolism
Calcium / metabolism*
Calcium Channels / metabolism
Calcium Signaling*
Caspases / antagonists & inhibitors,  metabolism*
Jurkat Cells
Membrane Proteins / metabolism
Neoplasm Proteins / metabolism
T-Lymphocytes / drug effects,  immunology*
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD95; 0/CH-11 anti-fas antibody, human; 0/Calcium Channels; 0/Membrane Proteins; 0/Neoplasm Proteins; 0/ORAI1 protein, human; 0/STIM1 protein, human; 7440-70-2/Calcium; EC 3.4.22.-/Caspases

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