| Caspase-dependent apoptosis of the HCT-8 epithelial cell line induced by the parasite Giardia intestinalis. | |
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MedLine Citation:
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PMID: 17714487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Giardia intestinalis is a flagellated protozoan which causes enteric disease worldwide. Giardia trophozoites infect epithelial cells of the proximal small intestine and can cause acute or chronic diarrhea. The mechanism of epithelial injury in giardiasis remains unknown. A number of enteric pathogens, including protozoan parasites, are able to induce enterocyte apoptosis. The aim of this work was to assess whether G. intestinalis strain WB clone C6 is able to induce apoptosis in the human intestinal epithelial cell line HCT-8, and to investigate the role of caspases in this process. Results demonstrated that the parasite induces cell apoptosis, as confirmed by DNA fragmentation analysis, detection of active caspase-3 and degradation of the caspase-3 substrate PARP [poly(ADP-ribose) polymerase]. Furthermore, G. intestinalis infection induces activation of both the intrinsic and the extrinsic apoptotic pathways, down-regulation of the antiapoptotic protein Bcl-2 and up-regulation of the proapoptotic Bax, suggesting a possible role for caspase-dependent apoptosis in the pathogenesis of giardiasis. |
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Authors:
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Maria Antonietta Panaro; Antonia Cianciulli; Vincenzo Mitolo; Carlo Ivan Mitolo; Angela Acquafredda; Olga Brandonisio; Pasqua Cavallo |
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Publication Detail:
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Type: Journal Article Date: 2007-08-20 |
Journal Detail:
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Title: FEMS immunology and medical microbiology Volume: 51 ISSN: 0928-8244 ISO Abbreviation: FEMS Immunol. Med. Microbiol. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-10-09 Completed Date: 2007-12-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9315554 Medline TA: FEMS Immunol Med Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 302-9 Citation Subset: IM |
Affiliation:
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Department of Human Anatomy and Histology, University of Bari, Bari, Italy. ma.panaro@anatomia.uniba.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Caspase 3 / metabolism* Cell Line, Tumor DNA Fragmentation Epithelial Cells / parasitology* Gene Expression Regulation Giardia lamblia / physiology* Humans Poly(ADP-ribose) Polymerases / metabolism Proto-Oncogene Proteins c-bcl-2 / biosynthesis, metabolism bcl-2-Associated X Protein / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3 |
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