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Caspase activation in transgenic mice with Alzheimer-like pathology: results from a pilot study utilizing the caspase inhibitor, Q-VD-OPh.
MedLine Citation:
PMID:  20057974     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Despite the wealth of evidence supporting the activation of caspases in Alzheimer's disease (AD), chronic administration of a caspase inhibitor has never been tested in any animal model system. The purpose of the current report was to identify a suitable animal model that displays caspase activation and cleavage of critical proteins associated with AD, and secondly, to undertake a pilot study utilizing the novel caspase inhibitor, quinolyl-valyl-O-methylaspartyl-[-2, 6-difluorophenoxy]-methyl ketone (Q-VD-OPh). Analysis of 12 month-old TgCRND8 mice, which represent an early-onset animal model for AD, indicated the activation of caspase-7 as well as the cleavage of tau and the amyloid precursor protein (APP). Having established that TgCRND8 mice represent a suitable model system to target caspases therapeutically, a prophylactic study was initiated utilizing Q-VD-OPh. Three month-old TgCRND8 mice were injected intraperitoneally three times a week for three months with 10 mg/kg Q-VD-OPh and compared to control mice injected with vehicle. Although there was no apparent effect on extracellular Abeta deposition, chronic treatment with Q-VD-OPh did prevent caspase-7 activation and limited the pathological changes associated with tau, including caspase cleavage. These preliminary findings suggest that further studies examining the utility of Q-VD-OPh as a potential therapeutic compound for the treatment of AD are warranted.
Authors:
Troy T Rohn; Polina Kokoulina; Cody R Eaton; Wayne W Poon
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Publication Detail:
Type:  Journal Article     Date:  2009-11-05
Journal Detail:
Title:  International journal of clinical and experimental medicine     Volume:  2     ISSN:  1940-5901     ISO Abbreviation:  Int J Clin Exp Med     Publication Date:  2009  
Date Detail:
Created Date:  2010-01-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101471010     Medline TA:  Int J Clin Exp Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  300-8     Citation Subset:  -    
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