| Caspase-3 and its inhibitor Ac-DEVD-CHO in rat lens epithelial cell apoptosis induced by hydrogen in vitro. | |
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MedLine Citation:
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PMID: 12890378 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the role of caspase-3 and its inhibitor Ac-DEVD-CHO in rat lens epithelial cell apoptosis induced by hydrogen peroxide (H(2)O(2)) in vitro. METHODS: Rat lenses were incubated in modified Eagle's medium containing 2 mmol/L H(2)O(2) to induce apoptosis in vitro. Apoptosis in lens epithelial cells was assessed by transmission electron microscopy and annexin V-propidium iodide (PI) double staining flow cytometry after 12, 24 and 48 h of incubation. The activity of caspase-3 was analyzed by western blotting. RESULTS: Observations under transmission electron microscopy revealed that 2 mmol/L H(2)O(2) could effectively induce lens epithelial cell apoptosis in vitro. Caspase-3 activity increased during cell apoptosis and the peak measurement occurred at 24 h after treatment with H(2)O(2). Cell apoptosis was blocked by caspase-3 inhibitor Ac-DEVD-CHO. CONCLUSIONS: The activation of caspase-3 plays an important role in executing apoptosis in H(2)O(2)-treated lens epithelial cells and in the formation of cataract. The caspase-3 inhibitor Ac-DEVD-CHO may effectively prevent lens epithelial cell apoptosis caused by oxidative injury. |
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Authors:
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Ke Yao; Kaijun Wang; Wen Xu; Zhaohui Sun; Xingchao Shentu; Peijin Qiu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chinese medical journal Volume: 116 ISSN: 0366-6999 ISO Abbreviation: Chin. Med. J. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-07-31 Completed Date: 2003-10-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7513795 Medline TA: Chin Med J (Engl) Country: China |
Other Details:
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Languages: eng Pagination: 1034-8 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China. xlren@zju.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Caspase 3 Caspases / antagonists & inhibitors*, metabolism, physiology* Cysteine Proteinase Inhibitors / pharmacology* Enzyme Activation Epithelial Cells / cytology Female Hydrogen Peroxide / pharmacology* Lens, Crystalline / cytology* Oligopeptides / pharmacology* Organ Culture Techniques Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Cysteine Proteinase Inhibitors; 0/Oligopeptides; 0/acetyl-aspartyl-glutamyl-valyl-aspartal; 7722-84-1/Hydrogen Peroxide; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
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