| Caspase-3 activation and apoptosis induction coupled with the retrograde transport of shiga toxin: inhibition by brefeldin A. | |
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MedLine Citation:
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PMID: 11118908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Caspase proteolytic activities, such as caspase-3, -2 and -6, of THP-1 human monocytic cells were markedly increased in a time- and dose-dependent manner by treatment with purified Shiga toxin 1 (Stx1) or Stx2. Caspase-3 activation was strictly correlated with internucleosomal DNA fragmentation and chromatin condensation of the cells. In addition, the specific caspase-3 inhibitor, Ac-DEVD-CHO, decreased the percentage of apoptotic cells. The purified B-subunit of Stx1 did not induce apoptosis in THP-1 cells. Caspase-3 activation, DNA fragmentation and chromatin condensation caused by Stx were completely blocked by pretreatment of cells with brefeldin A, an inhibitor of Golgi functions. The findings suggest that Stx1 as well as Stx2 activate caspase-3, which plays a critical role in apoptosis, and that the apoptotic signals rise after Stx is transported to the Golgi apparatus. |
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Authors:
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S Kojio; H Zhang; M Ohmura; F Gondaira; N Kobayashi; T Yamamoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS immunology and medical microbiology Volume: 29 ISSN: 0928-8244 ISO Abbreviation: FEMS Immunol. Med. Microbiol. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-01-12 Completed Date: 2001-04-05 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9315554 Medline TA: FEMS Immunol Med Microbiol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 275-81 Citation Subset: IM |
Affiliation:
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Department of Bacteriology, School of Medicine, Niigata University, Niigata, Japan. kojio@med.niigata-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Brefeldin A / pharmacology* Caspase 3 Caspases / metabolism* Cell Line Chromatin DNA Fragmentation Enzyme Activation / drug effects Humans Monocytes / cytology, drug effects*, enzymology Shiga Toxin 1 / metabolism*, pharmacology Shiga Toxin 2 / metabolism*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Shiga Toxin 1; 0/Shiga Toxin 2; 20350-15-6/Brefeldin A; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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