| Caspase-3 and -7 mediate apoptosis of human Chang's conjunctival cells induced by enterovirus 70. | |
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MedLine Citation:
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PMID: 16427675 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Enterovirus 70 (EV70) is the major etiological agent of acute hemorrhagic conjunctivitis (AHC). EV70 m.o.i.- (multiplicity of infection) and time-dependently induced apoptosis in human Chang's conjunctival (HCC) cells. UV- or heat-inactivated EV70 did not induce apoptosis. EV70-induced apoptosis was inhibited by cycloheximide and methoxysuccinyl-Ala-Ala-Pro-Val-chloromethylketone (MPCMK), but not actinomycin D and guanidine.HCl (although guanidine.HCl inhibited the apoptosis induced by EV70 infection at 0.5 PFU/cell for 18 h). EV70 infection induced activation of caspase-3 and -7 and degradation of the constitutively activated caspase-6. EV70-induced apoptotic DNA ladders and activated caspase-3 and -7, correlated with virus release. Caspase inhibitor IX (Z-VD-FMK) inhibited EV70-induced apoptosis and virus release, but not intracellular viral production. The results suggest that infectious virus and the syntheses of viral proteins especially EV70 proteases, but not viral genome RNA, are required for caspase-3 and -7-mediated EV70-induced apoptosis, and that apoptosis through cell lysis promotes EV70 release from HCC cells. |
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Authors:
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Dequan Chen; Donald E Texada; Chris Duggan; Youping Deng; Thomas B Redens; Marlyn P Langford |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-20 |
Journal Detail:
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Title: Virology Volume: 347 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-04-24 Completed Date: 2006-11-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 307-22 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130-3932, USA. dequan.chen@irrdallas.org |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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physiology* Caspase 3 Caspase 6 Caspases / metabolism* Cell Line Conjunctiva / cytology*, virology Enterovirus / physiology* Enterovirus Infections / immunology*, pathology Humans |
| Chemical | |
Reg. No./Substance:
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EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/CASP6 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 6; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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