| Caspase-14: analysis of gene structure and mRNA expression during keratinocyte differentiation. | |
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MedLine Citation:
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PMID: 11062009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Caspase-14 is expressed in a tissue-specific manner in mouse skin. Here we determined the complete caspase-14 cDNA sequence of human caspase-14 by rapid amplification of cDNA ends. Sequence comparison with a cosmid clone containing genomic DNA revealed that the human caspase-14 gene comprises seven exons. Facultative utilization of a cryptic splice acceptor site within intron 5 leads to the formation of two mRNA species. In situ hybridization of human skin showed that caspase-14 is expressed in the uppermost layer of living epidermal cells, i.e., the granular layer, in hair follicles and sebaceous glands. In vitro caspase-14 transcription was low in subconfluent cultures but strongly increased when keratinocyte differentiation was simulated by maintaining cells at confluence for several days. This transcriptional upregulation was suppressed in the presence of a high extracellular calcium concentration. Our findings show that caspase-14 is regulated at the level of transcription during keratinocyte differentiation in vitro and in vivo. |
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Authors:
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L Eckhart; J Ban; H Fischer; E Tschachler |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 277 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-11-17 Completed Date: 2000-12-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 655-9 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Academic Press. |
Affiliation:
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Division of Immunology, Allergy, and Infectious Diseases, University of Vienna Medical School, Vienna, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Caspase 14 Caspases / biosynthesis, genetics* Cell Differentiation / genetics* Cells, Cultured DNA / analysis Gene Expression Regulation, Enzymologic* Genome, Human Humans Keratinocytes / physiology* Molecular Sequence Data RNA Splicing RNA, Messenger / biosynthesis, metabolism Skin / cytology, enzymology, metabolism Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 9007-49-2/DNA; EC 3.4.22.-/CASP14 protein, human; EC 3.4.22.-/Casp14 protein, mouse; EC 3.4.22.-/Caspase 14; EC 3.4.22.-/Caspases |
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