| Caspase-1 activity is required for neuronal differentiation of PC12 cells: cross-talk between the caspase and calpain systems. | |
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MedLine Citation:
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PMID: 15843036 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previously, we have found that caspase-1 activity is increased during myoblast differentiation to myotubes. Here we show that caspase-1 activity is required for PC12 differentiation to neuronal-like cells. Caspase-1 is shown to be activated (by immunoblotting and by assessing activity in cell extracts) in the PC12 cells following the initial stage of differentiation. The inhibition of caspase-1 arrests PC12 cells at an intermediate stage of differentiation and prevents neurite outgrowth in these cells; the inhibition is reversed upon the removal of the inhibitor. Calpastatin (calpain endogenous specific inhibitor, and a known caspase substrate) is diminished at the later stages of PC12 cell differentiation, and diminution is prevented by caspase-1 inhibition. The degradation of fodrin (a known caspase and calpain substrate) is found in the advanced stage of differentiation. Caspase-1 has been implicated in the activation of proinflammatory cytokines, and in cell apoptosis. The involvement of caspase-1 in two distinct differentiation processes (myoblast fusion and neuronal differentiation of PC12 cells) indicates a function for this caspase in differentiation processes, and suggests some common mechanisms underlying caspase roles in such processes. |
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Authors:
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T Vaisid; N S Kosower; S Barnoy |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1743 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-04-21 Completed Date: 2005-06-09 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 223-30 Citation Subset: IM |
Affiliation:
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Department of Human Genetics and Molecular Medicine, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium-Binding Proteins / metabolism Calpain / antagonists & inhibitors, metabolism* Carrier Proteins / metabolism Caspase 1 / antagonists & inhibitors, metabolism* Cell Differentiation* Immunoblotting Microfilament Proteins / metabolism PC12 Cells Protease Inhibitors / pharmacology Rats |
| Chemical | |
Reg. No./Substance:
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0/Calcium-Binding Proteins; 0/Carrier Proteins; 0/Microfilament Proteins; 0/Protease Inhibitors; 0/fodrin; 79079-11-1/calpastatin; EC 3.4.22.-/Calpain; EC 3.4.22.36/Caspase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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