Document Detail


Case studies of the spatial heterogeneity of DNA viruses in the cystic fibrosis lung.
MedLine Citation:
PMID:  21980056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microbial communities in the lungs of patients with cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) have been shown to be spatially heterogeneous. Viral communities may also vary spatially, leading to localized viral populations and infections. Here, we characterized viral communities from multiple areas of the lungs of two patients with late-stage CF using metagenomics, that is, the explanted lungs from a transplant patient and lungs acquired postmortem. All regions harbored eukaryotic viruses that may infect the human host, notably herpesviruses, anelloviruses, and papillomaviruses. In the highly diseased apical lobes of explant lungs, viral diversity was extremely low, and only eukaryotic viruses were present. The absence of phage suggests that CF-associated microbial biofilms may escape top-down controls by phage predation. The phages present in other lobes of explant lungs and in all lobes of postmortem lungs comprised distinct communities, and encoded genes for clinically important microbial phenotypes, including small colony variants and antibiotic resistance. Based on the these observations, we postulate that viral communities in CF lungs are spatially distinct and contribute to CF pathology by augmenting the metabolic potential of resident microbes, as well as by directly damaging lung tissue via carcinomas and herpesviral outbreaks.
Authors:
Dana Willner; Matthew R Haynes; Mike Furlan; Nicole Hanson; Breeann Kirby; Yan Wei Lim; Paul B Rainey; Robert Schmieder; Merry Youle; Douglas Conrad; Forest Rohwer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-10-06
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  46     ISSN:  1535-4989     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-07     Completed Date:  2012-03-25     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  127-31     Citation Subset:  IM    
Affiliation:
Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, Queensland 4072, Australia. d.willner@uq.edu.au
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MeSH Terms
Descriptor/Qualifier:
Bacteriophages / genetics
Cystic Fibrosis / complications,  virology*
DNA Viruses / classification,  isolation & purification*
Drug Resistance, Microbial / genetics
Humans
Virus Diseases / complications
Grant Support
ID/Acronym/Agency:
R01 GM095384/GM/NIGMS NIH HHS; R01 GM095384-01/GM/NIGMS NIH HHS; R01 GM095384-01/GM/NIGMS NIH HHS
Comments/Corrections

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