Document Detail


Cartilage regeneration using mesenchymal stem cells and a PLGA-gelatin/chondroitin/hyaluronate hybrid scaffold.
MedLine Citation:
PMID:  16720040     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The study was to produce a novel hybrid poly-(lactic-co-glycolic acid) (PLGA)-gelatin/chondroitin/hyaluronate (PLGA-GCH) scaffold and evaluate its potentials in cartilage repair. The porous PLGA-GCH scaffold was developed to mimic the natural extra cellular matrix of cartilage. The differentiated mesenchymal stem cells (MSCs) seeded on PLGA-GCH or PLGA scaffold were incubated in vitro and showed that, compared to PLGA scaffold, the PLGA-GCH scaffold significantly augmented the proliferation of MSCs and GAG synthesis. Then autologous differentiated MSCs/PLGA-GCH was implanted to repair full-thickness cartilage defect in rabbit, while MSCs/PLGA for the contra lateral cartilage defect (n=30). Fifteen additional rabbits without treatment for defects were used as control. Histology observation showed the MSCs/PLGA-GCH repair group had better chondrocyte morphology, integration, continuous subchondral bone, and much thicker newly formed cartilage compared with MSCs/PLGA repair group 12 and 24 weeks postoperatively. There was a significant difference in histological grading score between these two groups, which both showed much better repair than control. The present study implied that the hybrid PLGA-GCH scaffold might serve as a new way to keep the differentiation of MSCs for enhancing cartilage repair.
Authors:
Hongbin Fan; Yunyu Hu; Chunli Zhang; Xusheng Li; Rong Lv; Ling Qin; Rui Zhu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-23
Journal Detail:
Title:  Biomaterials     Volume:  27     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-06-05     Completed Date:  2006-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  4573-80     Citation Subset:  IM    
Affiliation:
Institute of Orthopaedics & Traumatology, Xijing Hospital, the Fourth Military Medical University, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / chemistry*
Cartilage, Articular / cytology,  physiology*
Chondroitin / analogs & derivatives*,  chemistry
Guided Tissue Regeneration / methods
Hyaluronic Acid / analogs & derivatives*,  chemistry
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells / drug effects,  physiology
Polyglactin 910 / chemistry*
Rabbits
Regeneration*
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/poly-(lactic-co-glycolic acid)-gelatin chondroitin hyaluronate; 34346-01-5/Polyglactin 910; 9004-61-9/Hyaluronic Acid; 9007-27-6/Chondroitin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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