Document Detail


Cartilage polysaccharide induces apoptosis in human leukemia K562 cells.
MedLine Citation:
PMID:  17516137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we extracted a polysaccharide (short-chain polysaccharide [PS]) from porcine cartilage and examined its function in chronic myeloid leukaemia by using human K562 cells and mouse L1210 cells. Results of cell proliferation assay indicated that PS inhibited cancer cell growth at different concentrations, while it had little effect on normal cells. The presence of morphological aspects of apoptosis, such as nuclear shrinkage, was shown in H&E stained sections. The occurrence of PS-induced apoptosis was confirmed by TUNEL assay and cell cycle analysis. The results of immunofluorescent staining indicated the molecular mechanism underlying. Through interfering with the cell cycle of tumor cells, PS may induce apoptosis by downregulating the expression level of cyclin D1 and upregulating the level of p21 protein. Correlation analysis of apoptosis and MAPK suggested that inactivation of ERK was crucial for PS induced apoptosis, while JNK phosphorylation had a small effect and p38 was not involved. In vivo assay showed that PS inhibited L1210 cell growth in vivo and prolonged the life span of L1210-bearing mice. We conclude that PS is a polysaccharide with anticancer effects and induced apoptosis in human K562 cells.
Authors:
A-J Liu; W Song; N Yang; Y-J Liu; G-R Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-22
Journal Detail:
Title:  Cell biology and toxicology     Volume:  23     ISSN:  0742-2091     ISO Abbreviation:  Cell Biol. Toxicol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2008-01-01     Completed Date:  2008-03-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8506639     Medline TA:  Cell Biol Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  465-76     Citation Subset:  IM    
Affiliation:
Tianjin Key Laboratory of Food Nutrition and Safety, Institute of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, PR China. liuanjun666@yahoo.com.cn
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Cartilage / chemistry*
Cell Cycle / drug effects
Cell Proliferation / drug effects
Cyclin D1 / metabolism
Cyclin-Dependent Kinase Inhibitor p21 / metabolism
Fluorescent Antibody Technique
Humans
In Situ Nick-End Labeling
K562 Cells
Male
Mice
Mitogen-Activated Protein Kinases / metabolism
NIH 3T3 Cells
Polysaccharides / isolation & purification,  pharmacology*
Staining and Labeling
Swine
Chemical
Reg. No./Substance:
0/Cyclin-Dependent Kinase Inhibitor p21; 0/Polysaccharides; 136601-57-5/Cyclin D1; EC 2.7.11.24/Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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