Document Detail


Cartilage on the move: cartilage lineage tracing during tadpole metamorphosis.
MedLine Citation:
PMID:  23036161     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The reorganization of cranial cartilages during tadpole metamorphosis is a set of complex processes. The fates of larval cartilage-forming cells (chondrocytes) and sources of adult chondrocytes are largely unknown. Individual larval cranial cartilages may either degenerate or remodel, while many adult cartilages appear to form de novo during metamorphosis. Determining the extent to which adult chondrocytes/cartilages are derived from larval chondrocytes during metamorphosis requires new techniques in chondrocyte lineage tracing. We have developed two transgenic systems to label cartilage cells throughout the body with fluorescent proteins. One system strongly labels early tadpole cartilages only. The other system inducibly labels forming cartilages at any developmental stage. We examined cartilages of the skull (viscero- and neurocranium), and identified larval cartilages that either resorb or remodel into adult cartilages. Our data show that the adult otic capsules, tecti anterius and posterius, hyale, and portions of Meckel's cartilage are derived from larval chondrocytes. Our data also suggest that most adult cartilages form de novo, though we cannot rule out the potential for extreme larval chondrocyte proliferation or de- and re-differentiation, which could dilute our fluorescent protein signal. The transgenic lineage tracing strategies developed here are the first examples of inducible, skeleton-specific, lineage tracing in Xenopus.
Authors:
Ryan R Kerney; Alison L Brittain; Brian K Hall; Daniel R Buchholz
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-10-04
Journal Detail:
Title:  Development, growth & differentiation     Volume:  54     ISSN:  1440-169X     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-16     Completed Date:  2013-03-04     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  739-52     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors Development, Growth & Differentiation © 2012 Japanese Society of Developmental Biologists.
Affiliation:
Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Branchial Region / metabolism
Cartilage / cytology,  growth & development*,  metabolism*
Cell Differentiation
Cell Line
Cell Lineage
Chondrocytes / cytology,  physiology*
Chondrogenesis / physiology*
Doxycycline / pharmacology
Larva / growth & development,  metabolism
Metamorphosis, Biological*
Skull / cytology,  growth & development
Triiodothyronine / pharmacology
Xenopus laevis
Grant Support
ID/Acronym/Agency:
R03 5F32DK010069-03/DK/NIDDK NIH HHS; R03 HD059995/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
564-25-0/Doxycycline; 6893-02-3/Triiodothyronine
Comments/Corrections

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