Document Detail


Carryover test in frozen serum cored by an automated frozen sample aliquotter.
MedLine Citation:
PMID:  24836633     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A cleaning protocol for the CryoXtract Automated Frozen Sample Aliquotter (AFSA) was tested for its effectiveness in eliminating sample-to-sample carryover during hands-free frozen sample aliquotting. The instrument's test platform was used to extract a statistically significant number of frozen cores from frozen bovine serum samples spiked with human DNA and from identical DNA-free frozen controls, processed in alternating succession, with a proprietary cleaning protocol performed in between samples. Measurements of DNA content by a highly sensitive real-time quantitative polymerase chain reaction assay revealed no traces of DNA in the frozen control cores, whereas they revealed the expected DNA amounts in all DNA-containing frozen cores. Analysis of high-copy DNA demonstrated that CryoXtract's AFSA's cleaning protocol effectively reduced DNA carryover by more than a million-fold.
Authors:
Joseph V Geisberg; Todd Basque
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Publication Detail:
Type:  Journal Article     Date:  2011-11-11
Journal Detail:
Title:  Biopreservation and biobanking     Volume:  9     ISSN:  1947-5535     ISO Abbreviation:  Biopreserv Biobank     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2014-05-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101507284     Medline TA:  Biopreserv Biobank     Country:  United States    
Other Details:
Languages:  eng     Pagination:  373-7     Citation Subset:  -    
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