Document Detail


Carrier-mediated uptake of Levofloxacin by BeWo cells, a human trophoblast cell line.
MedLine Citation:
PMID:  19629508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Placental transfer of Levofloxacin (LF), a broad spectrum fluoroquinolone antibiotic, and its inhibition was investigated in BeWo cells, a human trophoblast cell line. METHODS: The experiments of LF uptake by BeWo cells were performed after preincubation and in the presence of the P-glycoprotein inhibitors (Cyclosporin A, Verapamil and Quercetin), the organic anion/cation transporter inhibitor (Cimetidine) and the MCT substrates (lactic acid and salicylic acid). RESULTS: P-glycoprotein inhibitors increased the uptake of LF by BeWo cells. The increase in LF accumulation by Cyclosporin A, Verapamil and Quercetin was by 30, 90 and 80%, respectively. Cimetidine, the organic cation inhibitor, increased the transport of LF by 48%. Lactic acid and salicylic acid, the MCT substrates, initially decreased the accumulation of LF by 30% and subsequently increased the uptake of LF by 500 and 53%, respectively. CONCLUSIONS: The uptake of LF by human trophoblast cells is mediated by multiple transporters as well as passive diffusion.
Authors:
Hana Polachek; Gershon Holcberg; Joseph Polachek; Mazal Rubin; Valeria Feinshtein; Eyal Sheiner; Zvi Ben-Zvi
Related Documents :
9331498 - Polymer-encapsulated pc-12 cells demonstrate high-affinity uptake of dopamine in vitro ...
6643338 - Interactions of antibiotics and phagocytes.
6892118 - Uptake of methylmercury and inorganic mercury by mouse glioma and mouse neuroblastoma c...
19661298 - Biotinylated poly(amido)amine (pamam) dendrimers as carriers for drug delivery to ovari...
9524568 - Inhibin gene expression in a large cell calcifying sertoli cell tumour and serum inhibi...
24514918 - Growth inhibitory in vitro effects of glycyrrhizic acid in u251 glioblastoma cell line.
Publication Detail:
Type:  Journal Article     Date:  2009-07-23
Journal Detail:
Title:  Archives of gynecology and obstetrics     Volume:  281     ISSN:  1432-0711     ISO Abbreviation:  Arch. Gynecol. Obstet.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-05     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8710213     Medline TA:  Arch Gynecol Obstet     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  833-8     Citation Subset:  IM    
Affiliation:
Department of Clinical Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, P.O. Box 653, 84105, Beer-Sheva, Israel. polache@bgu.ac.il
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacokinetics*
Cell Line, Tumor
Humans
Ion Pumps / antagonists & inhibitors,  metabolism
Ofloxacin / pharmacokinetics*
Trophoblasts / metabolism*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Ion Pumps; 82419-36-1/Ofloxacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Obstetric and perinatal outcome in HIV positive women receiving HAART in urban Nigeria.
Next Document:  Artifacts caused by cochlear implants with non-removable magnets in 3T MRI: phantom and cadaveric st...