| Carrier-mediated uptake of Levofloxacin by BeWo cells, a human trophoblast cell line. | |
| | |
MedLine Citation:
|
PMID: 19629508 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: Placental transfer of Levofloxacin (LF), a broad spectrum fluoroquinolone antibiotic, and its inhibition was investigated in BeWo cells, a human trophoblast cell line. METHODS: The experiments of LF uptake by BeWo cells were performed after preincubation and in the presence of the P-glycoprotein inhibitors (Cyclosporin A, Verapamil and Quercetin), the organic anion/cation transporter inhibitor (Cimetidine) and the MCT substrates (lactic acid and salicylic acid). RESULTS: P-glycoprotein inhibitors increased the uptake of LF by BeWo cells. The increase in LF accumulation by Cyclosporin A, Verapamil and Quercetin was by 30, 90 and 80%, respectively. Cimetidine, the organic cation inhibitor, increased the transport of LF by 48%. Lactic acid and salicylic acid, the MCT substrates, initially decreased the accumulation of LF by 30% and subsequently increased the uptake of LF by 500 and 53%, respectively. CONCLUSIONS: The uptake of LF by human trophoblast cells is mediated by multiple transporters as well as passive diffusion. |
| | |
Authors:
|
Hana Polachek; Gershon Holcberg; Joseph Polachek; Mazal Rubin; Valeria Feinshtein; Eyal Sheiner; Zvi Ben-Zvi |
Related Documents
:
|
9331498 - Polymer-encapsulated pc-12 cells demonstrate high-affinity uptake of dopamine in vitro ... 6643338 - Interactions of antibiotics and phagocytes. 6892118 - Uptake of methylmercury and inorganic mercury by mouse glioma and mouse neuroblastoma c... 19661298 - Biotinylated poly(amido)amine (pamam) dendrimers as carriers for drug delivery to ovari... 9524568 - Inhibin gene expression in a large cell calcifying sertoli cell tumour and serum inhibi... 2360458 - An immunohistochemical study of calcitonin-containing cells in benign and malignant thy... |
Publication Detail:
|
Type: Journal Article Date: 2009-07-23 |
Journal Detail:
|
Title: Archives of gynecology and obstetrics Volume: 281 ISSN: 1432-0711 ISO Abbreviation: Arch. Gynecol. Obstet. Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-04-05 Completed Date: 2010-06-29 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8710213 Medline TA: Arch Gynecol Obstet Country: Germany |
Other Details:
|
Languages: eng Pagination: 833-8 Citation Subset: IM |
Affiliation:
|
Department of Clinical Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, P.O. Box 653, 84105, Beer-Sheva, Israel. polache@bgu.ac.il |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Bacterial Agents
/
pharmacokinetics* Cell Line, Tumor Humans Ion Pumps / antagonists & inhibitors, metabolism Ofloxacin / pharmacokinetics* Trophoblasts / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Ion Pumps; 82419-36-1/Ofloxacin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Obstetric and perinatal outcome in HIV positive women receiving HAART in urban Nigeria.
Next Document: Artifacts caused by cochlear implants with non-removable magnets in 3T MRI: phantom and cadaveric st...