Document Detail

Carrier-mediated uptake of Levofloxacin by BeWo cells, a human trophoblast cell line.
MedLine Citation:
PMID:  19629508     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Placental transfer of Levofloxacin (LF), a broad spectrum fluoroquinolone antibiotic, and its inhibition was investigated in BeWo cells, a human trophoblast cell line. METHODS: The experiments of LF uptake by BeWo cells were performed after preincubation and in the presence of the P-glycoprotein inhibitors (Cyclosporin A, Verapamil and Quercetin), the organic anion/cation transporter inhibitor (Cimetidine) and the MCT substrates (lactic acid and salicylic acid). RESULTS: P-glycoprotein inhibitors increased the uptake of LF by BeWo cells. The increase in LF accumulation by Cyclosporin A, Verapamil and Quercetin was by 30, 90 and 80%, respectively. Cimetidine, the organic cation inhibitor, increased the transport of LF by 48%. Lactic acid and salicylic acid, the MCT substrates, initially decreased the accumulation of LF by 30% and subsequently increased the uptake of LF by 500 and 53%, respectively. CONCLUSIONS: The uptake of LF by human trophoblast cells is mediated by multiple transporters as well as passive diffusion.
Hana Polachek; Gershon Holcberg; Joseph Polachek; Mazal Rubin; Valeria Feinshtein; Eyal Sheiner; Zvi Ben-Zvi
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Publication Detail:
Type:  Journal Article     Date:  2009-07-23
Journal Detail:
Title:  Archives of gynecology and obstetrics     Volume:  281     ISSN:  1432-0711     ISO Abbreviation:  Arch. Gynecol. Obstet.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-05     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8710213     Medline TA:  Arch Gynecol Obstet     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  833-8     Citation Subset:  IM    
Department of Clinical Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, P.O. Box 653, 84105, Beer-Sheva, Israel.
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MeSH Terms
Anti-Bacterial Agents / pharmacokinetics*
Cell Line, Tumor
Ion Pumps / antagonists & inhibitors,  metabolism
Ofloxacin / pharmacokinetics*
Trophoblasts / metabolism*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Ion Pumps; 82419-36-1/Ofloxacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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