Document Detail


Carrier erythrocytes from cattle can be prepared from cells stored in a hypotonic, porous, dialyzed state.
MedLine Citation:
PMID:  3440057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hypotonic dialysis of bovine erythrocytes to 160-180 mosmol/kg produces cells with metastable pores. Cells stored for 3 weeks in a hypotonic dialyzed state can be used to prepare carrier erythrocytes. Pores generated by nonlytic hypotonic conditions are initially greater than 40 A in radius but shrink to less than 20 A with storage beyond 1 week. A metastable pore allows for hemoglobin isolation from hypotonically dialyzed cells.
Authors:
J R DeLoach; K Andrews
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  9     ISSN:  0885-4513     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-04-18     Completed Date:  1988-04-18     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  443-9     Citation Subset:  IM    
Affiliation:
Veterinary Toxicology and Entomology Research Laboratory, U.S. Department of Agriculture, College Station, Texas 77841.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Preservation*
Carbon Radioisotopes / diagnostic use
Cattle
Dialysis
Erythrocytes* / ultrastructure
Hypotonic Solutions
Inulin / blood
Microscopy, Electron, Scanning
Sucrose / blood
Tritium / diagnostic use
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Hypotonic Solutions; 10028-17-8/Tritium; 57-50-1/Sucrose; 9005-80-5/Inulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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