Document Detail


Carrier detection by DNA linkage analysis in eighty Thai hemophilia A families.
MedLine Citation:
PMID:  12403227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNA linkage analysis was performed in Thai hemophilia A families to evaluate its value for carrier detection. Both intragenic and extragenic polymorphic DNA regions of the factor VIII gene, including Bcl I-RFLP in intron 18, microsatellites (CA repeats) in introns 13 and 22, and extragenic Stl4 (DXS 52) VNTR, were amplified by polymerase chain reaction (PCR) before analyses by appropriate electrophoretic procedures. A total of 80 Thai hemophilia A families (48 with a family history and 32 with a sporadic case), containing 349 DNA samples from 90 hemophilia A patients, 143 parents, and 116 relatives, were analyzed. Heterozygosities in the patients' mothers from both families with a family history and with a sporadic case were observed in 71 out of 80 families (88.75%) for all polymorphic DNA markers analyzed. The carrier status could be identified in 36 females and excluded in 44 females. This result indicates that the DNA linkage analysis can be used for carrier detection or exclusion in the majority of Thai hemophilia A families. It should also be useful for prenatal diagnosis in families at risk of hemophilia A, which is part of the prevention and control of this disease.
Authors:
Chularatana Mahasandana; Parichat Pung-Amritt; Ajjima Treesucon; Siripan Petrarat; Gavivann Veerakul; Sukon Visudhiphan; Pa-Thai Yenchitsomanus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the Medical Association of Thailand = Chotmaihet thangphaet     Volume:  85 Suppl 2     ISSN:  0125-2208     ISO Abbreviation:  J Med Assoc Thai     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-10-29     Completed Date:  2002-11-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7507216     Medline TA:  J Med Assoc Thai     Country:  Thailand    
Other Details:
Languages:  eng     Pagination:  S513-21     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Adult
Base Sequence
Child
Child, Preschool
Cohort Studies
DNA Mutational Analysis
Female
Hemophilia A / epidemiology,  genetics*
Heterozygote*
Heterozygote Detection
Humans
Linkage (Genetics)*
Male
Molecular Sequence Data
Pedigree
Polymerase Chain Reaction / methods*
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length
Retrospective Studies
Thailand

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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