| Carotenoids induce apoptosis in the T-lymphoblast cell line Jurkat E6.1. | |
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MedLine Citation:
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PMID: 12180130 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Epidemiologically, a high-carotenoid intake via a fruit- and vegetable-rich diet is associated with a decreased risk of various forms of cancer. The mechanisms by which carotenoids exert this protective effect are controversial. In this study, we examined the potency of a range of carotenoids commonly found in human plasma to induce apoptosis in Jurkat E6.1 malignant T-lymphoblast cells. At a concentration of 20 microM, the order of potency to induce apoptosis after 24 h was: beta-carotene > lycopene > lutein > beta-cryptoxanthin = zeaxanthin. Canthaxanthin failed to induce apoptosis under these conditions. beta-Carotene induced apoptosis in a time- and concentration-dependent manner with a lowest effective concentration of about 3 microM. Pre-conditioning of beta-carotene for 72 h destroyed its pro-apoptotic activity almost completely, whereas degradation for 6 h or less did not, indicating that either beta-carotene itself and/or an early degradation product of beta-carotene are the death-inducing compounds. Apoptosis induced by beta-carotene was characterized by chromatin condensation and nuclear fragmentation, DNA degradation, PARP cleavage and caspase-3 activation. The antioxidant BO-653 inhibited the degradation of beta-carotene in vitro and significantly increased its cytotoxicity, indicating that a pro-oxidant effect of beta-carotene is unlikely to cause its pro-apoptotic activity. The induction of apoptosis in transformed cells by carotenoids may explain their protective effect against cancer formation in humans. Possible pathways for induction of apoptosis by carotenoids are discussed. |
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Authors:
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Karin Müller; Keri L H Carpenter; Iain R Challis; Jeremy N Skepper; Mark J Arends |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Free radical research Volume: 36 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-08-15 Completed Date: 2003-01-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
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Languages: eng Pagination: 791-802 Citation Subset: IM |
Affiliation:
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Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Blotting, Western Canthaxanthin / pharmacology Carotenoids / pharmacology Caspase 3 Caspases / metabolism Chemiluminescent Measurements Chromatin / metabolism Dose-Response Relationship, Drug Humans Jurkat Cells / drug effects, metabolism, pathology* Lutein / pharmacology Poly(ADP-ribose) Polymerases / metabolism Vitamin E / pharmacology Xanthophylls beta Carotene / analogs & derivatives*, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Xanthophylls; 127-40-2/Lutein; 1406-18-4/Vitamin E; 144-68-3/zeaxanthin; 36-88-4/Carotenoids; 472-70-8/cryptoxanthin; 502-65-8/lycopene; 514-78-3/Canthaxanthin; 7235-40-7/beta Carotene; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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