Document Detail


Carotenoid formation by Staphylococcus aureus.
MedLine Citation:
PMID:  5423369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The carotenoid pigments of Staphylococcus aureus U-71 were identified as phytoene; zeta-carotene; delta-carotene; phytofluenol; a phytofluenol-like carotenoid, rubixanthin; and three rubixanthin-like carotenoids after extraction, saponification, chromatographic separation, and determination of their absorption spectra. There was no evidence of carotenoid esters or glycoside ethers in the extract before saponification. During the aerobic growth cycle the total carotenoids increased from 45 to 1,000 nmoles per g (dry weight), with the greatest increases in the polar, hydroxylated carotenoids. During the anaerobic growth cycle, the total carotenoids increased from 20 nmoles per g (dry weight) to 80 nmoles per g (dry weight), and only traces of the polar carotenoids were formed. Light had no effect on carotenoid synthesis. About 0.14% of the mevalonate-2-(14)C added to the culture was incorporated into the carotenoids during each bacterial doubling. The total carotenoids did not lose radioactivity when grown in the absence of (14)C for 2.5 bacterial doublings. The total carotenoids did not lose radioactivity when grown in the absence of (14)C for 2.5 bacterial doublings. The incorporation and turnover of (14)C indicated the carotenes were sequentially desaturated and hydroxylated to form the polar carotenoids.
Authors:
R K Hammond; D C White
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  103     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1970 Jul 
Date Detail:
Created Date:  1970-08-10     Completed Date:  1970-08-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  191-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Autoradiography
Benzene
Carbon Isotopes
Carotenoids / analysis,  biosynthesis*,  isolation & purification
Chromatography, Paper
Culture Media
Lipids / isolation & purification
Mevalonic Acid / metabolism
Nitrogen
Oxygen
Petroleum
Spectrophotometry
Staphylococcus / analysis,  growth & development,  metabolism*
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Culture Media; 0/Lipids; 0/Petroleum; 150-97-0/Mevalonic Acid; 36-88-4/Carotenoids; 71-43-2/Benzene; 7727-37-9/Nitrogen; 7782-44-7/Oxygen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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