Document Detail


Carotenoid and fatty acid metabolism in light-stressed Dunaliella salina.
MedLine Citation:
PMID:  20229508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
beta-Carotene is overproduced in the alga Dunaliella salina in response to high light intensities. We have studied the effects of a sudden light increase on carotenoid and fatty acid metabolism using a flat panel photobioreactor that was run in turbidostat mode to ensure a constant light regime throughout the experiments. Upon the shift to an increased light intensity, beta-carotene production commenced immediately. The first 4 h after induction were marked by constant intracellular levels of beta-carotene (2.2 g LCV(-1)), which resulted from identical increases in the production rates of cell volume and beta-carotene. Following this initial phase, beta-carotene productivity continued to increase while the cell volume productivity dropped. As a result, the intracellular beta-carotene concentration increased reaching a maximum of 17 g LCV(-1) after 2 days of light stress. Approximately 1 day before that, the maximum beta-carotene productivity of 30 pg cell(-1) day(-1) (equivalent to 37 mg LRV(-1) day(-1)) was obtained, which was about one order of magnitude larger than the average productivity reported for a commercial beta-carotene production facility, indicating a vast potential for improvement. Furthermore, by studying the light-induced changes in both beta-carotene and fatty acid metabolism, it appeared that carotenoid overproduction was associated with oil globule formation and a decrease in the degree of fatty acid unsaturation. Our results indicate that cellular beta-carotene accumulation in D. salina correlates with accumulation of specific fatty acid species (C16:0 and C18:1) rather than with total fatty acid content.
Authors:
Packo P Lamers; Carlien C W van de Laak; Petrouchka S Kaasenbrood; Jeroen Lorier; Marcel Janssen; Ric C H De Vos; Raoul J Bino; René H Wijffels
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  106     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-26     Completed Date:  2010-08-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  638-48     Citation Subset:  IM    
Affiliation:
Wageningen University, Bioprocess Engineering, P.O. Box 8129, 6700 EV Wageningen, the Netherlands. packo.lamers@wur.nl
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MeSH Terms
Descriptor/Qualifier:
Biomass
Carotenoids / metabolism*
Fatty Acids / metabolism*
Light*
Stress, Physiological*
Volvocida / growth & development,  metabolism*,  radiation effects*
Chemical
Reg. No./Substance:
0/Fatty Acids; 36-88-4/Carotenoids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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