| Carotenoid and fatty acid metabolism in light-stressed Dunaliella salina. | |
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MedLine Citation:
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PMID: 20229508 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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beta-Carotene is overproduced in the alga Dunaliella salina in response to high light intensities. We have studied the effects of a sudden light increase on carotenoid and fatty acid metabolism using a flat panel photobioreactor that was run in turbidostat mode to ensure a constant light regime throughout the experiments. Upon the shift to an increased light intensity, beta-carotene production commenced immediately. The first 4 h after induction were marked by constant intracellular levels of beta-carotene (2.2 g LCV(-1)), which resulted from identical increases in the production rates of cell volume and beta-carotene. Following this initial phase, beta-carotene productivity continued to increase while the cell volume productivity dropped. As a result, the intracellular beta-carotene concentration increased reaching a maximum of 17 g LCV(-1) after 2 days of light stress. Approximately 1 day before that, the maximum beta-carotene productivity of 30 pg cell(-1) day(-1) (equivalent to 37 mg LRV(-1) day(-1)) was obtained, which was about one order of magnitude larger than the average productivity reported for a commercial beta-carotene production facility, indicating a vast potential for improvement. Furthermore, by studying the light-induced changes in both beta-carotene and fatty acid metabolism, it appeared that carotenoid overproduction was associated with oil globule formation and a decrease in the degree of fatty acid unsaturation. Our results indicate that cellular beta-carotene accumulation in D. salina correlates with accumulation of specific fatty acid species (C16:0 and C18:1) rather than with total fatty acid content. |
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Authors:
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Packo P Lamers; Carlien C W van de Laak; Petrouchka S Kaasenbrood; Jeroen Lorier; Marcel Janssen; Ric C H De Vos; Raoul J Bino; René H Wijffels |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 106 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-05-26 Completed Date: 2010-08-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 638-48 Citation Subset: IM |
Affiliation:
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Wageningen University, Bioprocess Engineering, P.O. Box 8129, 6700 EV Wageningen, the Netherlands. packo.lamers@wur.nl |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biomass Carotenoids / metabolism* Fatty Acids / metabolism* Light* Stress, Physiological* Volvocida / growth & development, metabolism*, radiation effects* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 36-88-4/Carotenoids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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