Document Detail

Carminic acid dye from the homopteran Dactylopius coccus hemolymph is consumed during treatment with different microbial elicitors.
MedLine Citation:
PMID:  12942514     Owner:  NLM     Status:  MEDLINE    
The activation of Dactylopius coccus (Costa) hemolymph with microbial polysaccharide molecules was studied. Hemolymph incubated in the presence of laminarin, zymosan, and N-acetyl glucosamine produced a dark fibrillar precipitated, and the red pigment (carminic acid) was consumed (measured spectrophotometrically at 495 nm). Lipopolysaccharide (LPS) did not induce any response. The reaction was inhibited with millimolar concentrations of serine and cysteine protease inhibitors, EGTA and phenyl thiourea. It was also diminished by prostaglandin synthesis inhibitors: dexamethasone, acetylsalicylic acid, and indomethacin. However, Mg2+ chelator EDTA did not inhibit hemolymph activation. Hemolymph proteins were depleted from soluble phase during treatment with laminarin, but a group of around 34 kDa remained unmodified. These results showed that D. coccus hemolymph is activated by microbial elicitors, its activation depends on eicosanoids, and suggest participation of a prophenoloxidase (PPO)-like activation system that could consume carminic acid. We are currently dissecting the molecular factors involved in D. coccus hemolymph activation to determine homologies and differences with other arthropods immune response pathways.
Fidel de la Cruz Hernández-Hernández; Fernando García-Gil de Muñoz; Alberto Rojas-Martínez; Salvador Hernández-Martínez; Humberto Lanz-Mendoza
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of insect biochemistry and physiology     Volume:  54     ISSN:  0739-4462     ISO Abbreviation:  Arch. Insect Biochem. Physiol.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-27     Completed Date:  2003-10-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8501752     Medline TA:  Arch Insect Biochem Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37-45     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Experimental Pathology Department, CINVESTAV-IPN, Madero, México DF.
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MeSH Terms
Acetylglucosamine / pharmacology*
Anticoagulants / metabolism
Carmine / analogs & derivatives*,  metabolism*
Catechol Oxidase / antagonists & inhibitors,  metabolism
Coloring Agents / metabolism*
Cyclooxygenase Inhibitors / pharmacology
Cysteine Proteinase Inhibitors / pharmacology
Electrophoresis, Polyacrylamide Gel
Enzyme Precursors / antagonists & inhibitors,  metabolism
Hemolymph / drug effects,  metabolism
Insect Proteins / metabolism
Insects / enzymology,  metabolism*
Polysaccharides / pharmacology
Polysaccharides, Bacterial / pharmacology*
Serine Proteinase Inhibitors / pharmacology
Zymosan / pharmacology*
Reg. No./Substance:
0/Anticoagulants; 0/Coloring Agents; 0/Cyclooxygenase Inhibitors; 0/Cysteine Proteinase Inhibitors; 0/Enzyme Precursors; 0/Insect Proteins; 0/Polysaccharides; 0/Polysaccharides, Bacterial; 0/Serine Proteinase Inhibitors; 1260-17-9/Carmine; 476-39-1/carminic acid; 7512-17-6/Acetylglucosamine; 9008-22-4/laminaran; 9010-72-4/Zymosan; EC 1.10.3.-/pro-phenoloxidase; EC Oxidase

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