Document Detail

Cardiovascular responses and neurotransmitter changes following blockade of nNOS within the ventrolateral medulla during static muscle contraction.
MedLine Citation:
PMID:  12788516     Owner:  NLM     Status:  MEDLINE    
Nitric oxide (NO) is synthesized from L-arginine through the activity of the synthetic enzyme, NO synthase (NOS). Previous studies have demonstrated the roles of the three isoforms of NOS, namely endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS) in cardiovascular regulation. However, no investigation has been done to study their individual role in modulating cardiovascular responses during static skeletal muscle contraction. In this study, we determined the effects of microdialyzing a specific nNOS antagonist into the rostral (RVLM) and caudal ventrolateral medulla (CVLM) on cardiovascular responses and glutamatergic/GABAergic neurotransmission during the exercise pressor reflex using rats. We hypothesized that the NO modulation of the exercise pressor reflex was largely influenced by specific nNOS activity within the ventrolateral medulla. Bilateral microdialysis of a selective nNOS antagonist, 1-(2-trifluoromethylphenyl)-imidazole (1.0 microM), for 30 or 60 min into the RVLM potentiated cardiovascular responses and glutamate release during a static muscle contraction. Levels of GABA within the RVLM were decreased. The cardiovascular responses and neurochemical changes to muscle contraction recovered following discontinuation of the drug. In contrast, bilateral application of the nNOS antagonist into CVLM attenuated cardiovascular responses and glutamate release during a static muscle contraction, but augmented GABA release. These results demonstrate that nNOS in the ventrolateral medulla plays an important role in modulating glutamatergic/GABAergic neurotransmission that regulates the exercise pressor reflex, and contributes to the sympathoexcitatory and sympathoinhibitory actions of NO within the RVLM and CVLM, respectively.
Takeshi Ishide; Surya M Nauli; Timothy J Maher; Ahmmed Ally
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  977     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-05     Completed Date:  2003-08-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  80-9     Citation Subset:  IM    
Department of Cardiovascular Science and Medicine, Chiba University School of Medicine, Chiba 260, Japan.
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MeSH Terms
Blood Pressure / drug effects
Brain Chemistry
Cardiovascular Physiological Phenomena*
Extracellular Space / metabolism
Glutamic Acid / metabolism
Heart Rate / drug effects
Imidazoles / pharmacology*
Medulla Oblongata / drug effects,  physiology*
Muscle Contraction / physiology*
Neurotransmitter Agents / metabolism*
Nitric Oxide Synthase / antagonists & inhibitors*
Nitric Oxide Synthase Type I
Rats, Sprague-Dawley
Time Factors
gamma-Aminobutyric Acid / metabolism
Reg. No./Substance:
0/Imidazoles; 0/Neurotransmitter Agents; 25371-96-4/1-(2-trifluoromethylphenyl)imidazole; 56-12-2/gamma-Aminobutyric Acid; 56-86-0/Glutamic Acid; EC Oxide Synthase; EC Oxide Synthase Type I; EC protein, rat

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