Document Detail


Cardiovascular effects induced by reticuline in normotensive rats.
MedLine Citation:
PMID:  15095148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cardiovascular effects of reticuline, isolated in a pure form from the stem of Ocotea duckei Vattimo, were studied in rats by using a combined in vivo and in vitro approach. In normotensive rats, reticuline (5, 10 and 20 mg/kg, i. v., randomly) injections produced an intense hypotension. This hypotensive response was attenuated after either, L-NAME (20 mg/kg, i. v.), a nitric oxide (NO) synthase inhibitor, or atropine (2 mg/kg, i. v.), a muscarinic receptor antagonist. In isolated rat aortic rings with intact endothelium, reticuline (3 x 10 ( - 6), 3 x 10 ( - 5), 3 x 10 ( - 4), 9 x 10 ( - 4) and 1.5 x 10 ( - 3) M) inhibited in a concentration-dependent manner the contractions induced by phenylephrine (1 microM), KCl (80 mM) and KCl (30 mM), [IC (50) = (0.4 +/- 0.1, 2.4 +/- 0.4 and 3 +/- 0.4) x 10 ( - 4) M, respectively). The effect of reticuline on phenylephrine-induced contractions was attenuated by removal of the vascular endothelium [IC (50) = (2.5 +/- 0.7) x 10 ( - 4) M]. Similar results were obtained after pretreatment of the rings with L-NAME 100 microM [IC (50) = (1.3 +/- 0.1) x 10 ( - 4) M], L-NAME 300 microM [IC (50) = (3 +/- 0.3) x 10 ( - 4) M] or atropine 1 microM [IC (50) = (1.2 +/- 0.2) x 10 ( - 4) M]. On the other hand, the effect of reticuline on phenylephrine-induced contractions was not affected by indomethacin 1 microM [IC (50) = (0.7 +/- 0.3) x 10 ( - 4) M]. Reticuline (3 x 10 ( - 6), 3 x 10 ( - 5), 3 x 10 ( - 4), 9 x 10 ( - 4) and 1.5 x 10 ( - 3) M) antagonized CaCl (2)-induced contractions, and also inhibited the intracellular calcium dependent transient contractions induced by norepinephrine (1 microM), but not those induced by caffeine (20 mM). These results suggest that the hypotensive effect of reticuline is probably due to a peripheral vasodilation in consequence of: 1) muscarinic stimulation and NOS activation in the vascular endothelium, 2) voltage-dependent Ca (2+) channel blockade and/or 3) inhibition of Ca (2+) release from norepinephrine-sensitive intracellular stores.
Authors:
Katy Lísias Dias; Celidarque Da Silva Dias; José Maria Barbosa-Filho; Reinaldo Nóbrega Almeida; Nadja De Azevedo Correia; Isac Almeida Medeiros
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta medica     Volume:  70     ISSN:  0032-0943     ISO Abbreviation:  Planta Med.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-19     Completed Date:  2004-06-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0066751     Medline TA:  Planta Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  328-33     Citation Subset:  IM    
Affiliation:
Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, João Pessoa-PB, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Alkaloids / administration & dosage,  pharmacology*,  therapeutic use
Animals
Aorta / drug effects
Benzylisoquinolines / administration & dosage,  pharmacology*,  therapeutic use
Blood Pressure / drug effects
Dose-Response Relationship, Drug
Endothelium, Vascular / drug effects
Infusions, Intravenous
Inhibitory Concentration 50
Muscle Contraction / drug effects*
Ocotea*
Phenylephrine / diagnostic use
Phytotherapy*
Plant Extracts / administration & dosage,  pharmacology,  therapeutic use
Plant Stems
Rats
Rats, Wistar
Vasodilator Agents / administration & dosage,  pharmacology*,  therapeutic use
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Benzylisoquinolines; 0/Plant Extracts; 0/Vasodilator Agents; 485-19-8/reticuline; 59-42-7/Phenylephrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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