Document Detail


Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb.
MedLine Citation:
PMID:  20084331     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the vascular responses and the blood pressure reducing effects of different fractions obtained from the methanol extract of Loranthus ferrugineus Roxb. (F. Loranthaceae). By means of solvent-solvent extraction, L. ferrugineus methanol extract (LFME) was successively fractionated with chloroform, ethyl acetate and n-butanol. The ability of these LFME fractions to relax vascular smooth muscle against phenylephrine (PE)- and KCl-induced contractions in isolated rat aortic rings was determined. In another set of experiments, LFME fractions were tested for blood pressure lowering activity in anesthetized adult male Sprague-Dawley rats (250-300 g, 14-18 weeks). The n-butanol fraction of LFME (NBF-LFME) produced a significant concentration-dependent inhibition of PE- and KCl-induced aortic ring contractions compared to other fractions. Moreover, NBF-LFME had a significantly higher relaxant effect against PE- than against high K+-induced contractions. In anesthetized Sprague-Dawley rats, NBF-LFME significantly lowered blood pressure in a dose-dependent manner and with a relatively longer duration of action compared to the other fractions. HPLC, UV and IR spectra suggested the presence of terpenoid constituents in both LFME and NBF-LFME. Accordingly, we conclude that NBF-LFME is the most potent fraction producing a concentration-dependent relaxation in vascular smooth muscle in vitro and a dose-dependent blood pressure lowering activity in vivo. The cardiovascular effects of NBF-LFME are most likely attributable to its terpenoid content.
Authors:
O Z Ameer; I M Salman; M J A Siddiqui; M F Yam; R N Sriramaneni; A Sadikun; Z Ismail; A M Shah; M Z Asmawi
Publication Detail:
Type:  Journal Article     Date:  2010-01-15
Journal Detail:
Title:  Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]     Volume:  43     ISSN:  1414-431X     ISO Abbreviation:  Braz. J. Med. Biol. Res.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-10     Completed Date:  2010-09-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8112917     Medline TA:  Braz J Med Biol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  186-94     Citation Subset:  IM    
Affiliation:
Department of Physiology and Pharmacology, Universiti Sains Malaysia, Penang, Malaysia. omar_3m@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
1-Butanol / isolation & purification,  pharmacology*
Animals
Aorta, Thoracic / drug effects
Blood Pressure / drug effects*
Chromatography, High Pressure Liquid
Loranthaceae / chemistry*
Male
Methanol / isolation & purification,  pharmacology
Muscle, Smooth, Vascular / drug effects*
Plant Extracts / pharmacology*
Rats
Rats, Sprague-Dawley
Vasodilation / drug effects*
Chemical
Reg. No./Substance:
0/Plant Extracts; 67-56-1/Methanol; 71-36-3/1-Butanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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