Document Detail

Cardiotin localization in mitochondria of cardiomyocytes in vivo and in vitro and its down-regulation during dedifferentiation.
MedLine Citation:
PMID:  18402829     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Cardiotin expression is observed in adult cardiac tissue. In the present study, we provide evidence for the specific localization of cardiotin in cardiac mitochondria and for its down-regulation during adaptive remodeling (dedifferentiation) of cardiomyocytes. METHODS: Immunocytochemistry was used to study cardiotin localization in adult rabbit papillary muscle, in late-stage embryonic rabbit left ventricular tissue, and in left ventricle samples of rabbits suffering from pressure and volume overload. Western blot analysis of cardiotin was performed in purified pig heart mitochondrial fractions. Cardiotin expression was monitored in vitro in isolated adult rat and rabbit left ventricular cardiomyocytes. RESULTS: Western blot analysis revealed the presence of cardiotin in the mitochondrial fractions of pig heart. Immunoelectron microscopy confirmed the presence of cardiotin in cardiac mitochondria of normal adult rabbits both in vivo and in vitro. Quantification of the localization of immunogold particles suggests an association of cardiotin with the mitochondrial inner membrane. Cardiotin expression is initiated in late-stage embryonic rabbit heart, whereas in adult ventricular tissue cardiotin clearly stained longitudinal arrays of mitochondria. Pressure- and volume-overloaded myocardium showed a reduction in cardiotin expression in dispersed local myocardial areas. Cell cultures of adult cardiomyocytes showed a gradual loss in cardiotin expression in parallel with a sarcomeric remodeling. CONCLUSIONS: Our results demonstrate the specific localization of cardiotin in adult cardiomyocyte mitochondria and propose its use as an early marker for cardiomyocyte adaptive remodeling and dedifferentiation.
Ronald B Driesen; Fons K Verheyen; Gert Schaart; Ann de Mazière; Christoph Viebahn; Frits W Prinzen; Marie-Hélène Lenders; Wiel Debie; Andreas Totzeck; Marcel Borgers; Frans C S Ramaekers
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Publication Detail:
Type:  Journal Article     Date:  2008-02-20
Journal Detail:
Title:  Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology     Volume:  18     ISSN:  1879-1336     ISO Abbreviation:  Cardiovasc. Pathol.     Publication Date:    2009 Jan-Feb
Date Detail:
Created Date:  2008-12-08     Completed Date:  2008-12-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9212060     Medline TA:  Cardiovasc Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19-27     Citation Subset:  IM    
Department of Molecular Cell Biology, School for Cardiovascular Diseases (CARIM), University of Maastricht, Maastricht, The Netherlands.
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MeSH Terms
Actinin / metabolism*
Aortic Valve Insufficiency / metabolism*,  pathology
Cell Dedifferentiation*
Cells, Cultured
Disease Models, Animal
Fluorescent Antibody Technique, Indirect
Heart / embryology
Heart Ventricles / chemistry,  metabolism,  pathology
Microscopy, Immunoelectron
Mitochondria / metabolism*,  ultrastructure
Myocytes, Cardiac / metabolism*,  pathology,  ultrastructure
Organ Specificity
Papillary Muscles / metabolism,  pathology,  ultrastructure
Reg. No./Substance:
0/cardiotin; 11003-00-2/Actinin

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