Document Detail

Cardioprotective effect of nicorandil in histidine-tryptophan-ketoglurate solution during the cold storage of isolated hearts.
MedLine Citation:
PMID:  8669099     Owner:  NLM     Status:  MEDLINE    
We compared the efficacy of using histidine-tryptophan-ketoglurate (HTK) solution with that of University of Wisconsin (UW) solution for heart preservation in an isolated rat heart preparation. Nicorandil (NCR) exerts its action as an ATP-sensitive potassium channel opener at low extracellular potassium concentrations, and HTK solution has a low potassium concentration. Therefore, we also investigated the efficacy of using HTK solution with NCR following 12-hr preservation. Hearts isolated from male Wistar rats were mounted on a Langendorff apparatus to estimate baseline aortic flow (AF), coronary flow (CF), cardiac out-put (CO), heart rate (HR), systolic pressure (SP), aortic mean pressure, and the rate-pressure product (RPP). The hearts were divided into four groups: group 1, 8-hr storage in UW solution; groups 2 and 3, 8- or 12-hr storage in HTK solution, respectively; and group 4, 12-hr storage in HTK solution with NCR. They were arrested and stored at 4 degrees C in each preservation solution. Following storage, they were reperfused and postpreservative function was measured to assess cardiac functional recovery. Concentrations of creatine phosphokinase, troponin-T, and lactate in the coronary perfusate were measured. Frozen tissue samples from groups 3 and 4 were analyzed for adenylate content and cGMP. The myocardial water content was also measured. The recovery of AF, CF, CO, SP, and RPP in group 2 was significantly improved compared with that in group 1 (P<0.05). The recovery of AF, CF, CO and HR in group 4 was significantly better than that in group 3 (P<0.05). Creatine phosphokinase leakage in group 2 and troponin-T leakage in group 4 were significantly reduced (P<0.05 vs. groups 1 and 3, respectively). Total adenine nucleotides and the adenylate energy charge in group 4 were well sustained (P<0.05 vs. group 3). These results suggest that HTK solution is more effective than UW solution for cardiac preservation, and that NCR provides still better protection.
K Gu; S Kin; Y Saitoh; S Nosaka; T Sasaki; M Yamauchi; K Nakayama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplantation     Volume:  61     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-08-02     Completed Date:  1996-08-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1572-5     Citation Subset:  IM    
First Department of Surgery, Shimane Medical University, Izumo, Japan.
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MeSH Terms
Adenosine / pharmacology
Allopurinol / pharmacology
Cardioplegic Solutions / pharmacology*
Cold Temperature
Cyclic GMP / analysis
Glucose / pharmacology
Glutathione / pharmacology
Heart / drug effects*,  physiology
Insulin / pharmacology
Mannitol / pharmacology
Niacinamide / analogs & derivatives*,  pharmacology
Organ Preservation*
Organ Preservation Solutions*
Potassium Chloride / pharmacology
Procaine / pharmacology
Raffinose / pharmacology
Rats, Wistar
Vasodilator Agents / pharmacology*
Reg. No./Substance:
0/Bretschneider cardioplegic solution; 0/Cardioplegic Solutions; 0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 0/Vasodilator Agents; 11061-68-0/Insulin; 315-30-0/Allopurinol; 50-99-7/Glucose; 512-69-6/Raffinose; 58-61-7/Adenosine; 59-46-1/Procaine; 65141-46-0/Nicorandil; 69-65-8/Mannitol; 70-18-8/Glutathione; 7447-40-7/Potassium Chloride; 7665-99-8/Cyclic GMP; 98-92-0/Niacinamide

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