Document Detail


Cardioprotection afforded by chronic exercise is mediated by the sarcolemmal, and not the mitochondrial, isoform of the KATP channel in the rat.
MedLine Citation:
PMID:  16223762     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was conducted to examine the role of myocardial ATP-sensitive potassium (K(ATP)) channels in exercise-induced protection from ischaemia-reperfusion (I-R) injury. Female rats were either sedentary (Sed) or exercised for 12 weeks (Tr). Hearts were excised and underwent a 1-2 h regional I-R protocol. Prior to ischaemia, hearts were subjected to pharmacological blockade of the sarcolemmal K(ATP) channel with HMR 1098 (SedHMR and TrHMR), mitochondrial blockade with 5-hydroxydecanoic acid (5HD; Sed5HD and Tr5HD), or perfused with buffer containing no drug (Sed and Tr). Infarct size was significantly smaller in hearts from Tr animals (35.4 +/- 2.3 versus 44.7 +/- 3.0% of the zone at risk for Tr and Sed, respectively). Mitochondrial K(ATP) blockade did not abolish the training-induced infarct size reduction (30.0 +/- 3.4 versus 38.0 +/- 2.6 in Tr5HD and Sed5HD, respectively); however, sarcolemmal K(ATP) blockade completely eradicated the training-induced cardioprotection. Infarct size was 71.2 +/- 3.3 and 64.0 +/- 2.4% of the zone at risk for TrHMR and Sed HMR. The role of sarcolemmal K(ATP) channels in Tr-induced protection was also supported by significant increases in both subunits of the sarcolemmal K(ATP) channel following training. LV developed pressure was better preserved in hearts from Tr animals, and was not influenced by addition of HMR 1098. 5HD decreased pressure development regardless of training status, from 15 min of ischaemia through the duration of the protocol. This mechanical dysfunction was likely to be due to a 5HD-induced increase in myocardial Ca2+ content following I-R. The major findings of the present study are: (1) unlike all other known forms of delayed cardioprotection, infarct sparing following chronic exercise was not abolished by 5HD; (2) pharmacological blockade of the sarcolemmal K(ATP) channel nullified the cardioprotective benefits of exercise training; and (3) increased expression of sarcolemmal K(ATP) channels was observed following chronic training.
Authors:
David A Brown; Adam J Chicco; Korinne N Jew; Micah S Johnson; Joshua M Lynch; Peter A Watson; Russell L Moore
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2005-10-13
Journal Detail:
Title:  The Journal of physiology     Volume:  569     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-16     Completed Date:  2006-05-04     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  913-24     Citation Subset:  IM    
Affiliation:
Department of Integrative Physiology, 202D Carlson Gymnasium, Campus Box 354, Boulder, CO 80309 USA.
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MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters
Animals
Benzamides / pharmacology
Calcium / metabolism
Decanoic Acids / pharmacology
Female
Heart / drug effects
Hydroxy Acids / pharmacology
Myocardial Infarction / metabolism*,  pathology,  prevention & control
Myocardial Reperfusion Injury / metabolism*,  pathology,  prevention & control
Myocardium / metabolism
Physical Conditioning, Animal
Potassium Channel Blockers / pharmacology
Potassium Channels / drug effects,  metabolism*
Potassium Channels, Inwardly Rectifying / metabolism
Protein Isoforms / metabolism
Rats
Rats, Sprague-Dawley
Receptors, Drug
Sarcolemma / drug effects,  metabolism*
Ventricular Dysfunction, Left / prevention & control
Grant Support
ID/Acronym/Agency:
GM066728-01/GM/NIGMS NIH HHS; HL40306/HL/NHLBI NIH HHS; HL72790/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Benzamides; 0/Decanoic Acids; 0/HMR 1098; 0/Hydroxy Acids; 0/Kir6.2 channel; 0/Potassium Channel Blockers; 0/Potassium Channels; 0/Potassium Channels, Inwardly Rectifying; 0/Protein Isoforms; 0/Receptors, Drug; 0/mitochondrial K(ATP) channel; 0/sulfonylurea receptor; 624-00-0/5-hydroxydecanoic acid; 7440-70-2/Calcium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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