| Cardiomyocyte cell cycle control and growth estimation in vivo--an analysis based on cardiomyocyte nuclei. | |
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MedLine Citation:
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PMID: 20071355 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: Adult mammalian cardiomyocytes are traditionally viewed as being permanently withdrawn from the cell cycle. Whereas some groups have reported none, others have reported extensive mitosis in adult myocardium under steady-state conditions. Recently, a highly specific assay of 14C dating in humans has suggested a continuous generation of cardiomyocytes in the adult, albeit at a very low rate. Mice represent the most commonly used animal model for these studies, but their short lifespan makes them unsuitable for 14C studies. Herein, we investigate the cellular growth pattern for murine cardiomyocyte growth under steady-state conditions, addressed with new analytical and technical strategies, and we furthermore relate this to gene expression patterns. METHODS AND RESULTS: The observed levels of DNA synthesis in early life were associated with cardiomyocyte proliferation. Mitosis was prolonged into early life, longer than the most conservative previous estimates. DNA synthesis in neonatal life was attributable to bi-nucleation, therefore suggesting that cardiomyocytes withdraw from the cell cycle shortly after birth. No cell cycle activity was observed in adult cardiomyocytes and significant polyploidy was observed in cardiomyocyte nuclei. CONCLUSION: Gene analyses identified 32 genes whose expression was predicted to be particular to day 3-4 neonatal myocytes, compared with embryonic or adult cells. These cell cycle-associated genes are crucial to the understanding of the mechanisms of bi-nucleation and physiological cellular growth in the neonatal period. |
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Authors:
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Stuart Walsh; Annica Pont?n; Bernd K Fleischmann; Stefan Jovinge |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-13 |
Journal Detail:
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Title: Cardiovascular research Volume: 86 ISSN: 1755-3245 ISO Abbreviation: Cardiovasc. Res. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-13 Completed Date: 2010-06-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0077427 Medline TA: Cardiovasc Res Country: England |
Other Details:
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Languages: eng Pagination: 365-73 Citation Subset: IM |
Affiliation:
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Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund SE-22184, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging Animals Animals, Newborn Bromodeoxyuridine / metabolism Cell Cycle* / genetics Cell Nucleus / metabolism, physiology* Cell Proliferation* Cell Separation / methods Cells, Cultured DNA Replication* Flow Cytometry Gene Expression Regulation, Developmental Gestational Age Green Fluorescent Proteins / biosynthesis, genetics Immunohistochemistry Ki-67 Antigen / metabolism Kinetics Mice Mice, Inbred C57BL Mice, Inbred DBA Mice, Transgenic Mitosis Myocytes, Cardiac / metabolism, physiology* Polyploidy Reverse Transcriptase Polymerase Chain Reaction Troponin T / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Ki-67 Antigen; 0/Troponin T; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 59-14-3/Bromodeoxyuridine |
| Comments/Corrections | |
Comment In:
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Cardiovasc Res. 2010 Jun 1;86(3):347-8
[PMID:
20375104
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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