Document Detail


Cardiomyocyte enlargement, proliferation and maturation during chronic fetal anaemia in sheep.
MedLine Citation:
PMID:  19700519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chronic anaemia increases the workload of the growing fetal heart, leading to cardiac enlargement. To determine which cellular process increases cardiac mass, we measured cardiomyocyte sizes, binucleation as an index of terminal differentiation, and tissue volume fractions in hearts from control and anaemic fetal sheep. Fourteen chronically catheterized fetal sheep at 129 days gestation had blood withdrawn for 9 days to cause severe anaemia; 14 control fetuses were of similar age. At postmortem examination, hearts were either enzymatically dissociated or fixed for morphometric analysis. Daily isovolumetric haemorrhage reduced fetal haematocrit from a baseline value of 35% to 15% on the final day (P < 0.001). At the study conclusion, anaemic fetuses had lower arterial pressures than control fetuses (P < 0.05). Heart weights were increased by 39% in anaemic fetuses compared with control hearts (P < 0.0001), although the groups had similar body weights; the heart weight difference was not due to increased ventricular wall water content or disproportionate non-myocyte tissue expansion. Cardiomyocytes from anaemic fetuses tended to be larger than those of control fetuses. There were no statistically significant differences between groups in the cardiomyocyte cell cycle activity. The degree of terminal differentiation was greater in the right ventricle of anaemic compared with control fetuses by 8% (P < 0.05). Anaemia substantially increased heart weight in fetal sheep. The volume proportions of connective and vascular tissue were unchanged. Cardiomyocyte mass expanded by a balanced combination of cellular enlargement, increased terminal differentiation and accelerated proliferation.
Authors:
Sonnet S Jonker; M Kathryn Giraud; George D Giraud; Natasha N Chattergoon; Samantha Louey; Lowell E Davis; J Job Faber; Kent L Thornburg
Related Documents :
8602319 - Umbilical venous doppler velocity pulsations and inferior vena cava pressure elevations...
9249119 - Maternal and fetal effects of isoproterenol in the gravid ewe.
3419739 - Fetal vibro-acoustic stimulation: magnitude and duration of fetal heart rate accelerati...
8769729 - Endothelin-1 constricts fetoplacental microcirculation and decreases fetal o2 consumpti...
9736799 - Correlation of change in uterine activity to blood loss in the third stage of labour.
8590189 - Uterine flow velocity waveform shape as an indicator of maternal and placental developm...
8814389 - Evaluation of the tracking potential of a noninvasive estimator of cardiac output.
16005099 - Walking for exercise--does three times per week influence risk factors in type 2 diabetes?
10821779 - Tissue angiotensin ii and endothelin-1 modulate differently the response to flow in mes...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-08-21
Journal Detail:
Title:  Experimental physiology     Volume:  95     ISSN:  1469-445X     ISO Abbreviation:  Exp. Physiol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-12     Completed Date:  2010-08-04     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  9002940     Medline TA:  Exp Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  131-9     Citation Subset:  IM    
Affiliation:
Oregon Health & Science University, 3303 SW Bond Avenue CH14T, Portland, OR 97239-4501, USA. jonkers@ohsu.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Anemia / blood,  pathology*
Animals
Cell Enlargement*
Cell Proliferation*
Chronic Disease
Disease Models, Animal*
Female
Fetal Diseases / blood,  pathology*
Myocytes, Cardiac / metabolism,  pathology*
Pregnancy
Sheep
Grant Support
ID/Acronym/Agency:
P01 HD034430/HD/NICHD NIH HHS; P01 HD034430-15/HD/NICHD NIH HHS; P01HD34430/HD/NICHD NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Centrally-evoked increase in adrenal sympathetic outflow elicits immediate secretion of adrenaline i...
Next Document:  Vagus nerve stimulation inhibits the increase in Ca2+ transient and left ventricular force caused by...