Document Detail


Cardiac troponin in ischemic cardiomyocytes: Intracellular decrease before onset of cell death.
MedLine Citation:
PMID:  24607416     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIM: Cardiac troponin I (cTnI) and T (cTnT) are the most important biomarkers in the diagnosis of acute myocardial infarction (AMI). Nevertheless, they can be elevated in the absence of AMI. It is unclear if such elevations represent irreversible cardiomyocyte-damage or leakage from viable cardiomyocytes. Our objective is to evaluate whether cTn is released from viable cardiomyocytes in response to ischemia and to identify differences in the release of cTn and its molecular forms.
METHODS AND RESULTS: HL-1 cardiomyocytes (mouse) were subjected to ischemia (modeled by anoxia with glucose deprivation). The total contents and molecular forms of cTn were determined in culture media and cell lysates. Cell viability was assessed from the release of lactate dehydrogenase (LDH). Before the release of LDH, the intracellular cTn content in ischemic cells decreased significantly compared to control (52% for cTnI; 23% for cTnT) and was not matched by a cTn increase in the medium. cTnI decreased more rapidly than cTnT, resulting in an intracellular cTnT/cTnI ratio of 25.5 after 24h of ischemia. Western blots revealed changes in the relative amounts of fragmented cTnI and cTnT in ischemic cells.
CONCLUSIONS: HL-1 cardiomyocytes subjected to simulated ischemia released cTnI and cTnT only in combination with the release of LDH. We find no evidence of cTn release from viable cardiomyocytes, but did observe a significant decrease in cTn content, before the onset of cell death. Intracellular decrease of cTn in viable cardiomyocytes can have important consequences for the interpretation of cTn values in clinical practice.
Authors:
Alexander S Streng; Leo H J Jacobs; Robert W Schwenk; Eline P M Cardinaels; Steven J R Meex; Will K W H Wodzig; Marja P van Dieijen-Visser
Related Documents :
11515916 - Delayed presentation of right ventricular bullet embolus.
6694416 - Surgical considerations in the treatment of cardiac myxoma.
12086386 - Left ventricular thrombi in the presence of normal left ventricular function.
21960726 - Role of cardiac stem cells in cardiac pathophysiology: a paradigm shift in human myocar...
10469966 - Effect of posterior pericardiotomy on postoperative supraventricular arrhythmias and la...
22399856 - Outcome of the horizons-ami trial: bivalirudin enhances long-term survival in patients ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-4
Journal Detail:
Title:  Experimental and molecular pathology     Volume:  -     ISSN:  1096-0945     ISO Abbreviation:  Exp. Mol. Pathol.     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370711     Medline TA:  Exp Mol Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014. Published by Elsevier Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Innovative application of ionic liquid to separate Al and cathode materials from spent high-power li...
Next Document:  Unraveling the novel anti-osteosarcoma function of coptisine and its mechanisms.