Document Detail

Cardiac troponin T circulates in the free, intact form in patients with kidney failure.
MedLine Citation:
PMID:  16410339     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The clinical significance of the increased concentrations of cardiac troponins observed in patients with end stage renal disease (ESRD) in the absence of an acute coronary syndrome (ACS) is controversial. One proposed explanation is that immunoreactive fragments of cardiac troponin T (cTnT) accumulate in ESRD. We used gel-filtration chromatography (GFC) to ascertain whether fragments of cTnT, which could cross-react in the commercial diagnostic immunoassay (Roche Diagnostics), were the cause of the increased cTnT in the serum of patients with ESRD. METHODS: We subjected sera from ESRD patients (n = 21) receiving dialysis and having increased cTnT concentrations to size-separation GFC. We detected cTnT in the chromatography fractions by use of the same antibodies used in the commercial assay for serum cTnT. RESULTS: In all patients, cTnT immunoreactivity eluted as a major, homogeneous peak in an identical position between the peaks of serum prolactin [relative molecular mass (Mr) 23,000] and albumin (Mr 67,000): the elution pattern of cTnT in samples obtained from ACS patients was identical to that of the ESRD patients. There was no evidence that low-molecular-mass (Mr < 23,000) cTnT fragments were the cause of the increased cTnT in the patients studied. CONCLUSIONS: The form of cTnT observed in the serum of patients with kidney failure and immunoreactive in the diagnostic assay is predominantly the free intact form, as in patients with ACS. Our data are consistent with the view that circulating cTnT in renal failure reflects cardiac pathology.
Michael N Fahie-Wilson; David J Carmichael; Michael P Delaney; Paul E Stevens; Elizabeth M Hall; Edmund J Lamb
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-12
Journal Detail:
Title:  Clinical chemistry     Volume:  52     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-02     Completed Date:  2006-04-13     Revised Date:  2007-02-02    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  414-20     Citation Subset:  IM    
Department of Clinical Biochemistry, Southend Hospital, Westcliff-on-Sea, United Kingdom.
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MeSH Terms
Chromatography, Gel
Cross Reactions
Kidney Failure, Chronic / blood*
Protein Binding
Troponin T / blood*
Reg. No./Substance:
0/Troponin T
Comment In:
Clin Chem. 2006 Dec;52(12):2306-7; author reply 2307-9   [PMID:  17138853 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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