| Cardiac troponin T circulates in the free, intact form in patients with kidney failure. | |
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MedLine Citation:
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PMID: 16410339 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The clinical significance of the increased concentrations of cardiac troponins observed in patients with end stage renal disease (ESRD) in the absence of an acute coronary syndrome (ACS) is controversial. One proposed explanation is that immunoreactive fragments of cardiac troponin T (cTnT) accumulate in ESRD. We used gel-filtration chromatography (GFC) to ascertain whether fragments of cTnT, which could cross-react in the commercial diagnostic immunoassay (Roche Diagnostics), were the cause of the increased cTnT in the serum of patients with ESRD. METHODS: We subjected sera from ESRD patients (n = 21) receiving dialysis and having increased cTnT concentrations to size-separation GFC. We detected cTnT in the chromatography fractions by use of the same antibodies used in the commercial assay for serum cTnT. RESULTS: In all patients, cTnT immunoreactivity eluted as a major, homogeneous peak in an identical position between the peaks of serum prolactin [relative molecular mass (Mr) 23,000] and albumin (Mr 67,000): the elution pattern of cTnT in samples obtained from ACS patients was identical to that of the ESRD patients. There was no evidence that low-molecular-mass (Mr < 23,000) cTnT fragments were the cause of the increased cTnT in the patients studied. CONCLUSIONS: The form of cTnT observed in the serum of patients with kidney failure and immunoreactive in the diagnostic assay is predominantly the free intact form, as in patients with ACS. Our data are consistent with the view that circulating cTnT in renal failure reflects cardiac pathology. |
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Authors:
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Michael N Fahie-Wilson; David J Carmichael; Michael P Delaney; Paul E Stevens; Elizabeth M Hall; Edmund J Lamb |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-12 |
Journal Detail:
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Title: Clinical chemistry Volume: 52 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-03-02 Completed Date: 2006-04-13 Revised Date: 2007-02-02 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: United States |
Other Details:
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Languages: eng Pagination: 414-20 Citation Subset: IM |
Affiliation:
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Department of Clinical Biochemistry, Southend Hospital, Westcliff-on-Sea, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gel Cross Reactions Humans Immunoassay Kidney Failure, Chronic / blood* Protein Binding Troponin T / blood* |
| Chemical | |
Reg. No./Substance:
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0/Troponin T |
| Comments/Corrections | |
Comment In:
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Clin Chem. 2006 Dec;52(12):2306-7; author reply 2307-9
[PMID:
17138853
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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