Document Detail


Cardiac troponin T circulates in the free, intact form in patients with kidney failure.
MedLine Citation:
PMID:  16410339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The clinical significance of the increased concentrations of cardiac troponins observed in patients with end stage renal disease (ESRD) in the absence of an acute coronary syndrome (ACS) is controversial. One proposed explanation is that immunoreactive fragments of cardiac troponin T (cTnT) accumulate in ESRD. We used gel-filtration chromatography (GFC) to ascertain whether fragments of cTnT, which could cross-react in the commercial diagnostic immunoassay (Roche Diagnostics), were the cause of the increased cTnT in the serum of patients with ESRD. METHODS: We subjected sera from ESRD patients (n = 21) receiving dialysis and having increased cTnT concentrations to size-separation GFC. We detected cTnT in the chromatography fractions by use of the same antibodies used in the commercial assay for serum cTnT. RESULTS: In all patients, cTnT immunoreactivity eluted as a major, homogeneous peak in an identical position between the peaks of serum prolactin [relative molecular mass (Mr) 23,000] and albumin (Mr 67,000): the elution pattern of cTnT in samples obtained from ACS patients was identical to that of the ESRD patients. There was no evidence that low-molecular-mass (Mr < 23,000) cTnT fragments were the cause of the increased cTnT in the patients studied. CONCLUSIONS: The form of cTnT observed in the serum of patients with kidney failure and immunoreactive in the diagnostic assay is predominantly the free intact form, as in patients with ACS. Our data are consistent with the view that circulating cTnT in renal failure reflects cardiac pathology.
Authors:
Michael N Fahie-Wilson; David J Carmichael; Michael P Delaney; Paul E Stevens; Elizabeth M Hall; Edmund J Lamb
Related Documents :
19638709 - Comparative impact of scintigraphic parameters and b-type natriuretic peptide for the p...
15774989 - How well does b-type natriuretic peptide predict death and cardiac events in patients w...
21072029 - The role of b-type natriuretic peptide testing in patients with acute coronary syndromes.
19926599 - Red cell distribution width: an inexpensive and powerful prognostic marker in heart fai...
21691869 - Zinc deficiency in patients undergoing pancreatoduodenectomy for periampullary tumors i...
20177019 - Cross-sectional study of imatinib plasma trough levels in patients with advanced gastro...
20082199 - Abnormal levels of circulating endothelial progenitor cells during exacerbations of copd.
7135279 - Pulmonary sarcoidosis with an alveolar radiographic pattern.
2309629 - Value of electrophysiologic testing in patients with previous myocardial infarction and...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-12
Journal Detail:
Title:  Clinical chemistry     Volume:  52     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-02     Completed Date:  2006-04-13     Revised Date:  2007-02-02    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  414-20     Citation Subset:  IM    
Affiliation:
Department of Clinical Biochemistry, Southend Hospital, Westcliff-on-Sea, United Kingdom.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chromatography, Gel
Cross Reactions
Humans
Immunoassay
Kidney Failure, Chronic / blood*
Protein Binding
Troponin T / blood*
Chemical
Reg. No./Substance:
0/Troponin T
Comments/Corrections
Comment In:
Clin Chem. 2006 Dec;52(12):2306-7; author reply 2307-9   [PMID:  17138853 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  What about the remaining twins since single-embryo transfer? How far can (should) we go?
Next Document:  Minimal disease detection and confirmation in hematologic malignancies: combining cell sorting with ...