Document Detail

Cardiac-specific immunoenzymometric assay of troponin I in the early phase of acute myocardial infarction.
MedLine Citation:
PMID:  8504566     Owner:  NLM     Status:  MEDLINE    
The screening by immunoenzymometric assay (IEMA) of 784 monoclonal antibody (MAb) combinations resulted in the selection of an optimal pair of MAbs for measuring human cardiac troponin I (TnI). Using a one-step IEMA described here, we were able to detect TnI within the range of 0.2-20 micrograms/L in 30 min at room temperature. No cross-reactivity was observed with the skeletal isoforms of troponin up to a concentration of 500 micrograms/L. This assay was used to measure cardiac TnI in the plasma of 43 patients with acute myocardial infarction (AMI). TnI was detected relatively early after the onset of chest pain (4.3 +/- 2.1 h, mean +/- SD); the peak occurred after 12.2 +/- 4.6 h in a population that had undergone fibrinolysis. TnI disappearance was generally observed between 5 and 9 days after the onset of chest pain. No cardiac TnI could be detected in 145 healthy donors or in a control group of 6 patients (with skeletal damage or rhabdomyolysis). This assay allows a specific diagnosis of AMI in its early acute phase, with a high diagnostic specificity and sensitivity.
C Larue; C Calzolari; J P Bertinchant; F Leclercq; R Grolleau; B Pau
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  39     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-07-07     Completed Date:  1993-07-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  972-9     Citation Subset:  IM    
Sanofi Recherche, Montpellier, France.
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MeSH Terms
Antibodies, Monoclonal
Antibody Specificity
Antigens / immunology
Hydrogen-Ion Concentration
Immunoenzyme Techniques* / standards,  statistics & numerical data
Middle Aged
Muscles / chemistry
Myocardial Infarction / blood*,  diagnosis,  drug therapy
Myocardium / chemistry
Thrombolytic Therapy
Troponin / blood*,  immunology
Troponin I
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens; 0/Troponin; 0/Troponin I

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