| Cardiac progenitor cells and biotinylated insulin-like growth factor-1 nanofibers improve endogenous and exogenous myocardial regeneration after infarction. | |
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MedLine Citation:
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PMID: 19704095 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Cardiac progenitor cells (CPCs) possess the insulin-like growth factor-1 (IGF-1)-IGF-1 receptor system, and IGF-1 can be tethered to self-assembling peptide nanofibers (NF-IGF-1), leading to prolonged release of this growth factor to the myocardium. Therefore, we tested whether local injection of clonogenic CPCs and NF-IGF-1 potentiates the activation and differentiation of delivered and resident CPCs enhancing cardiac repair after infarction. METHODS AND RESULTS: Myocardial infarction was induced in rats, and untreated infarcts and infarcts treated with CPCs or NF-IGF-1 only and CPCs and NF-IGF-1 together were analyzed. With respect to infarcts exposed to CPCs or NF-IGF-1 alone, combination therapy resulted in a greater increase in the ratio of left ventricular mass to chamber volume and a better preservation of +dP/dt, -dP/dt, ejection fraction, and diastolic wall stress. Myocardial regeneration was detected in all treated infarcts, but the number of newly formed myocytes with combination therapy was 32% and 230% higher than with CPCs and NF-IGF-1, respectively. Corresponding differences in the volume of regenerated myocytes were 48% and 115%. Similarly, the length density of newly formed coronary arterioles with both CPCs and NF-IGF-1 was 73% and 83% greater than with CPCs and NF-IGF-1 alone, respectively. Importantly, activation of resident CPCs by paracrine effects contributed to cardiomyogenesis and vasculogenesis. Collectively, CPCs and NF-IGF-1 therapy reduced infarct size more than CPCs and NF-IGF-1 alone. CONCLUSIONS: The addition of nanofiber-mediated IGF-1 delivery to CPC therapy improved in part the recovery of myocardial structure and function after infarction. |
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Authors:
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M Elena Padin-Iruegas; Yu Misao; Michael E Davis; Vincent F M Segers; Grazia Esposito; Tomotake Tokunou; Konrad Urbanek; Toru Hosoda; Marcello Rota; Piero Anversa; Annarosa Leri; Richard T Lee; Jan Kajstura |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-08-24 |
Journal Detail:
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Title: Circulation Volume: 120 ISSN: 1524-4539 ISO Abbreviation: Circulation Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-09 Completed Date: 2009-10-07 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0147763 Medline TA: Circulation Country: United States |
Other Details:
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Languages: eng Pagination: 876-87 Citation Subset: AIM; IM |
Affiliation:
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Department of Anesthesia and Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Animals Apoptosis Biotin* Cell Fusion Cell Proliferation Cells, Cultured Coronary Vessels / physiopathology Female Insulin-Like Growth Factor I / administration & dosage* Myocardial Infarction / pathology, physiopathology*, surgery* Myocardium / pathology Myocytes, Cardiac* / pathology Nanostructures* Rats Rats, Inbred F344 Regeneration* / drug effects Stem Cell Transplantation* Stem Cells* Tissue Survival Ventricular Function |
| Grant Support | |
ID/Acronym/Agency:
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EB003805/EB/NIBIB NIH HHS; R01 HL065573-07/HL/NHLBI NIH HHS; R01 HL075480-05/HL/NHLBI NIH HHS; R21 HL094894-01/HL/NHLBI NIH HHS; R21 HL094894-01S1/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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58-85-5/Biotin; 67763-96-6/Insulin-Like Growth Factor I |
| Comments/Corrections | |
Comment In:
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Circulation. 2009 Sep 8;120(10):831-4
[PMID:
19704092
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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